Detailed information of TA system TA168312

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3581891..3582618
• Replicon: chromosome
• Accession: NZ_CP059125
• Organism: Escherichia coli strain EC45

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: H0X93_RS17255
• Protein ID: WP_000547564.1
• Coordinates: 3582307..3582618 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H0X93_RS17250
• Protein ID: WP_000126294.1
• Coordinates: 3581891..3582310 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0X93_RS17230 (3576941)	3576941..3577468	-	528	WP_001078777.1	electron transport protein HydN	-
H0X93_RS17235 (3577617)	3577617..3578627	-	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
H0X93_RS17240 (3578887)	3578887..3580344	+	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
H0X93_RS17245 (3580353)	3580353..3581777	+	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
H0X93_RS17250 (3581891)	3581891..3582310	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
H0X93_RS17255 (3582307)	3582307..3582618	-	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
H0X93_RS17260 (3582792)	3582792..3583553	-	762	WP_001026446.1	hypothetical protein	-
H0X93_RS17265 (3583578)	3583578..3584048	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
H0X93_RS17270 (3584041)	3584041..3584451	-	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
H0X93_RS17275 (3584448)	3584448..3585215	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
H0X93_RS17280 (3585215)	3585215..3585757	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
H0X93_RS17285 (3585767)	3585767..3587476	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T168312 WP_000547564.1 NZ_CP059125:c3582618-3582307 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T168312 NZ_CP075717:101516-101914 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGTGTCAGGGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGCATCAGTTCGGTCACTCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT168312 WP_000126294.1 NZ_CP059125:c3582310-3581891 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT168312 NZ_CP075717:101289-101516 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCTGCCGGAGAGACATGGGACGAATGGTTCGACGGAA
ACAGTGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCTGGTATGCAGGAAAGGGAGTCATTCTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5