Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3865917..3866458 Replicon chromosome
Accession NZ_CP059119
Organism Escherichia coli strain Ec42

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag H0X94_RS18705 Protein ID WP_000615983.1
Coordinates 3866180..3866458 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag H0X94_RS18700 Protein ID WP_000729703.1
Coordinates 3865917..3866177 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0X94_RS18680 (3861592) 3861592..3862377 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
H0X94_RS18685 (3862349) 3862349..3864061 + 1713 Protein_3673 flagellar biosynthesis protein FlhA -
H0X94_RS18690 (3864285) 3864285..3864782 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
H0X94_RS18695 (3864958) 3864958..3865707 - 750 WP_000056849.1 C40 family peptidase -
H0X94_RS18700 (3865917) 3865917..3866177 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
H0X94_RS18705 (3866180) 3866180..3866458 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
H0X94_RS18710 (3866614) 3866614..3867354 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
H0X94_RS18715 (3867325) 3867325..3868092 - 768 WP_000333380.1 class II glutamine amidotransferase -
H0X94_RS18720 (3868298) 3868298..3868876 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island ant(3'')-Ia / blaOXA-1 / catA1 gmhA/lpcA 3806774..3875000 68226


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T168280 WP_000615983.1 NZ_CP059119:3866180-3866458 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T168280 NZ_CP059119:3866180-3866458 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT168280 WP_000729703.1 NZ_CP059119:3865917-3866177 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT168280 NZ_CP059119:3865917-3866177 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References