Detailed information of TA system TA168279

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3859720..3860414
• Replicon: chromosome
• Accession: NZ_CP059119
• Organism: Escherichia coli strain Ec42

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: H0X94_RS18665
• Protein ID: WP_001263489.1
• Coordinates: 3859720..3860118 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: H0X94_RS18670
• Protein ID: WP_000554758.1
• Coordinates: 3860121..3860414 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3855308)	3855308..3855388	-	81	NuclAT_13	-	-
- (3855308)	3855308..3855388	-	81	NuclAT_13	-	-
- (3855308)	3855308..3855388	-	81	NuclAT_13	-	-
- (3855308)	3855308..3855388	-	81	NuclAT_13	-	-
H0X94_RS18640 (3855984)	3855984..3856442	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
H0X94_RS18645 (3856703)	3856703..3858160	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
H0X94_RS18650 (3858217)	3858217..3858738	-	522	Protein_3666	peptide chain release factor H	-
H0X94_RS18655 (3858734)	3858734..3858940	-	207	Protein_3667	RtcB family protein	-
H0X94_RS18660 (3859258)	3859258..3859710	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
H0X94_RS18665 (3859720)	3859720..3860118	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
H0X94_RS18670 (3860121)	3860121..3860414	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
H0X94_RS18675 (3860466)	3860466..3861521	-	1056	WP_001226164.1	DNA polymerase IV	-
H0X94_RS18680 (3861592)	3861592..3862377	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
H0X94_RS18685 (3862349)	3862349..3864061	+	1713	Protein_3673	flagellar biosynthesis protein FlhA	-
H0X94_RS18690 (3864285)	3864285..3864782	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	ant(3'')-Ia / blaOXA-1 / catA1	gmhA/lpcA	3806774..3875000	68226

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T168279 WP_001263489.1 NZ_CP059119:c3860118-3859720 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T168279 NZ_CP075697:3819232-3819567 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT168279 WP_000554758.1 NZ_CP059119:c3860414-3860121 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT168279 NZ_CP075697:3818984-3819232 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3