Detailed information of TA system TA168263

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 813626..814353
• Replicon: chromosome
• Accession: NZ_CP059119
• Organism: Escherichia coli strain Ec42

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A829L2T9
• Locus tag: H0X94_RS03955
• Protein ID: WP_001521139.1
• Coordinates: 814042..814353 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H0X94_RS03950
• Protein ID: WP_000126294.1
• Coordinates: 813626..814045 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0X94_RS03930 (808711)	808711..809238	-	528	WP_001078777.1	electron transport protein HydN	-
H0X94_RS03935 (809387)	809387..810397	-	1011	WP_015912547.1	DNA-binding transcriptional regulator AscG	-
H0X94_RS03940 (810657)	810657..812114	+	1458	WP_020234027.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
H0X94_RS03945 (812123)	812123..813547	+	1425	WP_020234028.1	6-phospho-beta-glucosidase AscB	-
H0X94_RS03950 (813626)	813626..814045	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
H0X94_RS03955 (814042)	814042..814353	-	312	WP_001521139.1	type II toxin-antitoxin system HigB family toxin	Toxin
H0X94_RS03960 (814520)	814520..814990	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
H0X94_RS03965 (814983)	814983..815393	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
H0X94_RS03970 (815390)	815390..816157	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
H0X94_RS03975 (816157)	816157..816699	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
H0X94_RS03980 (816709)	816709..818418	-	1710	WP_001521140.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12493.23 Da
• Isoelectric Point: 9.4783

>T168263 WP_001521139.1 NZ_CP059119:c814353-814042 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T168263 NZ_CP075691:3471866-3472267 [Streptomyces sp. 135]
GTGATCTACCTCGACTCCTGCGCCCTGCTCAAGCTCGTCCTGCGGGAGGCCGAGACGGACGCATTGCGCACCTTCCTGGC
CGCCAGGGCGGCGGAGGGCCATGTGACCTCGGCACTGTCGCAAACCGAGGTGTTCCGTACGCTGGTGCGGGCCGGAGCGG
ATCCTGAAGTGGGTGACGCCGCCGAGGCGTTGCTCGACCGGCTGCTCCGCATCCGCGTCACGGATCCCGTGCTGCGGGCG
GCCGGCATGTTCCCGGCCCGCCACCTCCGCAGCCTGGACGCGATCCACCTGGCGAGCGCCGAGTACCTGGAACACGCGCT
CACGGCGTTCGTCACGTACGACAAGCGCCTGGCCGCCGCCGCGCTCGACCGCGGCCTCCCGGTCGAAGCGCCGGGCGCCT
AG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT168263 WP_000126294.1 NZ_CP059119:c814045-813626 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT168263 NZ_CP075691:3471573-3471869 [Streptomyces sp. 135]
ATGACCACCCGCGTACCCATCCGCCAGCTCCAGCAGCACGCCAGTGAACTCATCGACCGTGTCGCCGCGGGCGAGCGCGT
GGAGATCACTCGCAACGGCCGCCTGATCGCGGTGCTCGGACCTCCGGACCCGGAACAGCGCGTCATGGAAGACCTGGTCC
GCACCGGAACCGTCGACCCGGACAACGCGGCTTCGGCGCGCGGGCTCGGCGACTGGGAGCCTCTGGCCGCCCACCCGGGA
GCCTCCTCGAAGCTGTCCGAAGTCCTGTTGCGGATGCGCGAGGAAGAGGACCGGTGA

Structures

Toxin

• AlphaFold DB: A0A829L2T9