Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 604799..605598 Replicon chromosome
Accession NZ_CP059119
Organism Escherichia coli strain Ec42

Toxin (Protein)


Gene name yhaV Uniprot ID D3GWU5
Locus tag H0X94_RS02930 Protein ID WP_000347272.1
Coordinates 604799..605263 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag H0X94_RS02935 Protein ID WP_001307405.1
Coordinates 605263..605598 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0X94_RS02900 (599800) 599800..600234 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
H0X94_RS02905 (600252) 600252..601130 - 879 WP_001298314.1 PTS N-acetylgalactosamine transporter subunit IID -
H0X94_RS02910 (601120) 601120..601899 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
H0X94_RS02915 (601910) 601910..602383 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
H0X94_RS02920 (602406) 602406..603686 - 1281 WP_001521382.1 tagatose-bisphosphate aldolase subunit KbaZ -
H0X94_RS02925 (603935) 603935..604744 + 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
H0X94_RS02930 (604799) 604799..605263 - 465 WP_000347272.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H0X94_RS02935 (605263) 605263..605598 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H0X94_RS02940 (605747) 605747..607318 - 1572 WP_001273738.1 galactarate dehydratase -
H0X94_RS02945 (607693) 607693..609027 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
H0X94_RS02950 (609043) 609043..609813 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17832.26 Da        Isoelectric Point: 9.6924

>T168261 WP_000347272.1 NZ_CP059119:c605263-604799 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Download         Length: 465 bp

>T168261 NZ_CP075688:c40228-39947 [Pseudomonas amygdali pv. lachrymans]
ATGCTGCCGGTGGTCTGGCTCAGTCCCGCACTGGATGATCTGCGCGAAATCGCAACCTACATCGCATGGGAAAACCCGAG
CGCAGCGCGCCGGTTGAAAAGCCTCCTGCAGGAGGCTATCGAGCCGGTAGCCGAACATCCCTATTTATATCGTTCTGGGC
GAGCGCCAGGCACTCGCGAGCTGGTGGCTCACCCAAACTACGTCCTGGTCTATCGTGTCACCCTCAAGCGCATCGAGGTC
GTGAACGTGATCCACGCCAGACAGGAATACCCCTCTCCCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT168261 WP_001307405.1 NZ_CP059119:c605598-605263 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT168261 NZ_CP075688:c40416-40228 [Pseudomonas amygdali pv. lachrymans]
ATGGCCAACCTCTCACCCATCGTTTCAGAATTCGAAACCGATGAACAGGCCGCAAGCTACGACCGCTGGTTTCGCGCCAA
GGTCGCCGCCAGTCTGGCCGATCCGCGTCCGGCCATCCCTCACGATGAGGTCATGGCGGAAATAGAAGCGCTGATCGATC
AGGTTGAAACCCAACGTGAGAAACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829KUD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References