Detailed information of TA system TA168196

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 919634..920214
• Replicon: chromosome
• Accession: NZ_CP059060
• Organism: Synechococcus sp. LTW-R

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: H0O22_RS05215
• Protein ID: WP_185187915.1
• Coordinates: 919634..919912 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H0O22_RS05220
• Protein ID: WP_185187916.1
• Coordinates: 919912..920214 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0O22_RS05195	915363..916724	+	1362	WP_185187911.1	glutathione-disulfide reductase	-
H0O22_RS05200	916729..917436	+	708	WP_185187912.1	SDR family NAD(P)-dependent oxidoreductase	-
H0O22_RS05205	917433..918764	-	1332	WP_185187913.1	sodium:alanine symporter family protein	-
H0O22_RS05210	918814..919491	-	678	WP_185187914.1	DUF3386 domain-containing protein	-
H0O22_RS05215	919634..919912	+	279	WP_185187915.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H0O22_RS05220	919912..920214	+	303	WP_185187916.1	HigA family addiction module antidote protein	Antitoxin
H0O22_RS05225	920219..923512	-	3294	WP_185187917.1	carbamoyl-phosphate synthase large subunit	-
H0O22_RS05230	923640..924275	+	636	WP_185187918.1	DUF3318 domain-containing protein	-
H0O22_RS05235	924268..924681	+	414	WP_185187919.1	ribosome silencing factor	-
H0O22_RS05240	924694..925206	+	513	WP_185187920.1	CGLD27 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (25-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (19-71)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10757.23 Da
• Isoelectric Point: 8.5613

>T168196 WP_185187915.1 NZ_CP059060:919634-919912 [Synechococcus sp. LTW-R]
VIRSFRCADTERLAHGWAIPRFQAFERVARRKLRQLDIASRLEDLRIPPGNRLEALRGDRAGEHSIRINDQFRLCFIWSD
SGPEAVEIVDYH

Nucleotide

• Length: 279 bp

>T168196 NZ_CP075652:4774347-4774450 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10910.59 Da
• Isoelectric Point: 5.8146

>AT168196 WP_185187916.1 NZ_CP059060:919912-920214 [Synechococcus sp. LTW-R]
MTATHRLSPITPGELLVEEFLVPLGVSQYRLSKAIKVPASRISEIVSGQRSITADTDLRLCRFLGLSPGYWLRAQAAYDT
EIAGAELAEEIAKIQPLVNS

Nucleotide

• Length: 303 bp

>AT168196 NZ_CP075652:c4774488-4774217 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT