Detailed information of TA system TA168160

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 107652..108253
• Replicon: chromosome
• Accession: NZ_CP059045
• Organism: Cronobacter sakazakii strain 5563_17

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A6L3P9G2
• Locus tag: HZZ03_RS00505
• Protein ID: WP_012126103.1
• Coordinates: 107652..107963 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HZZ03_RS00510
• Protein ID: WP_007871962.1
• Coordinates: 107963..108253 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZZ03_RS00490	103010..104977	-	1968	WP_012126106.1	glycoside hydrolase family 127 protein	-
HZZ03_RS00495	104989..106392	-	1404	WP_012126105.1	MFS transporter	-
HZZ03_RS00500	106653..107477	+	825	WP_012126104.1	AraC family transcriptional regulator	-
HZZ03_RS00505	107652..107963	+	312	WP_012126103.1	hypothetical protein	Toxin
HZZ03_RS00510	107963..108253	+	291	WP_007871962.1	helix-turn-helix domain-containing protein	Antitoxin
HZZ03_RS00515	108327..109256	-	930	WP_007871963.1	formate dehydrogenase accessory protein FdhE	-
HZZ03_RS00520	109253..109888	-	636	WP_004385165.1	formate dehydrogenase cytochrome b556 subunit	-
HZZ03_RS00525	109885..110787	-	903	WP_004385164.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12070.99 Da
• Isoelectric Point: 10.0150

>T168160 WP_012126103.1 NZ_CP059045:107652-107963 [Cronobacter sakazakii]
MLFIETEIFTRAVTALLSDDDYQRLQWFLALRPDYGDVIPDTGGLRKARWLSGGRGKRGGVRIIYFYRPAADEIRLLLIY
RKGIKDDLSPHEKSVLRMLNERW

Nucleotide

• Length: 312 bp

>T168160 NZ_CP075649:2556603-2557925 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CAGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTAACGTTATTACCCGAAAA
TGAAACCATAACGCGCCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGGCTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTTAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCTGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCGTTTTTGATGGGGTCCAGCGAGGCGCTTCGCGATCGTTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTAAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10659.23 Da
• Isoelectric Point: 10.7279

>AT168160 WP_007871962.1 NZ_CP059045:107963-108253 [Cronobacter sakazakii]
MDKALFEQLTESLRQMNDISEGRREPSRVFVIDALKIKAIREASGLSQTKFASLISVSVDTLRNWEQGRRSPTGPARALL
RAIANDPQHVLSALAL

Nucleotide

• Length: 291 bp

>AT168160 NZ_CP075649:2556337-2556603 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACACTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A6L3P9G2