Detailed information of TA system TA168151

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 160935..161189
• Replicon: plasmid pSY3626C1_315k
• Accession: NZ_CP059044
• Organism: Escherichia coli strain SY3626C1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: HZ987_RS23460
• Protein ID: WP_001351576.1
• Coordinates: 160935..161141 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	161128..161189 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZ987_RS23430	156644..157090	-	447	Protein_166	DUF3363 domain-containing protein	-
HZ987_RS23435	157395..158099	-	705	WP_001067858.1	IS6-like element IS26 family transposase	-
HZ987_RS23440	158163..158288	-	126	Protein_168	antitoxin	-
HZ987_RS23445	159254..160111	-	858	WP_039002220.1	incFII family plasmid replication initiator RepA	-
HZ987_RS23450	160104..160178	-	75	WP_061357229.1	RepA leader peptide Tap	-
HZ987_RS23455	160410..160667	-	258	WP_001541895.1	replication regulatory protein RepA	-
HZ987_RS23460	160935..161141	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	161128..161189	+	62	NuclAT_1	-	Antitoxin
-	161128..161189	+	62	NuclAT_1	-	Antitoxin
-	161128..161189	+	62	NuclAT_1	-	Antitoxin
-	161128..161189	+	62	NuclAT_1	-	Antitoxin
HZ987_RS23465	161531..161743	-	213	WP_001541896.1	ANR family transcriptional regulator	-
HZ987_RS23470	161878..162438	-	561	WP_000139310.1	fertility inhibition protein FinO	-
HZ987_RS23475	162493..163236	-	744	WP_039002216.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(X4) / floR / qnrS1 / blaTEM-1B / ant(3'')-Ia / aac(3)-IId / lnu(F) / sul3 / aph(3')-Ia / sul2 / tet(M) / lnu(G)	-	1..315984	315984

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T168151 WP_001351576.1 NZ_CP059044:c161141-160935 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T168151 NZ_CP075649:c649673-649209 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAACCCCATTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT168151 NZ_CP059044:161128-161189 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin