Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 649731..650425 Replicon chromosome
Accession NZ_CP059043
Organism Escherichia coli strain SY3626C1

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag HZ987_RS03015 Protein ID WP_001263489.1
Coordinates 650027..650425 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag HZ987_RS03010 Protein ID WP_000554758.1
Coordinates 649731..650024 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZ987_RS02990 645363..645860 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
HZ987_RS02995 646084..647823 - 1740 WP_001334802.1 flagellar biosynthesis protein FlhA -
HZ987_RS03000 647768..648553 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
HZ987_RS03005 648624..649679 + 1056 WP_001226164.1 DNA polymerase IV -
HZ987_RS03010 649731..650024 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
HZ987_RS03015 650027..650425 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
HZ987_RS03020 650435..650887 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
HZ987_RS03025 651205..651411 + 207 Protein_590 RtcB family protein -
HZ987_RS03030 651407..651928 + 522 Protein_591 peptide chain release factor H -
HZ987_RS03035 651985..653442 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
HZ987_RS03040 653703..654161 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 654757..654837 + 81 NuclAT_12 - -
- 654757..654837 + 81 NuclAT_12 - -
- 654757..654837 + 81 NuclAT_12 - -
- 654757..654837 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T168117 WP_001263489.1 NZ_CP059043:650027-650425 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T168117 NZ_CP075640:103423-103674 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT168117 WP_000554758.1 NZ_CP059043:649731-650024 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT168117 NZ_CP075640:103671-103925 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References