Detailed information of TA system TA168093

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4191060..4191859
• Replicon: chromosome
• Accession: NZ_CP059003
• Organism: Escherichia coli strain 2D

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: H0E72_RS20100
• Protein ID: WP_000347273.1
• Coordinates: 4191395..4191859 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: V0TI39
• Locus tag: H0E72_RS20095
• Protein ID: WP_021547164.1
• Coordinates: 4191060..4191395 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0E72_RS20080	4186845..4187615	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
H0E72_RS20085	4187631..4188965	-	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
H0E72_RS20090	4189340..4190911	+	1572	WP_001273756.1	galactarate dehydratase	-
H0E72_RS20095	4191060..4191395	+	336	WP_021547164.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
H0E72_RS20100	4191395..4191859	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
H0E72_RS20105	4191914..4192723	-	810	WP_000072171.1	aga operon transcriptional regulator AgaR	-
H0E72_RS20110	4192972..4194252	+	1281	WP_000681958.1	tagatose-bisphosphate aldolase subunit KbaZ	-
H0E72_RS20115	4194275..4194748	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
H0E72_RS20120	4194759..4195538	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
H0E72_RS20125	4195528..4196406	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
H0E72_RS20130	4196424..4196858	+	435	WP_000948834.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4169331..4191859	22528

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T168093 WP_000347273.1 NZ_CP059003:4191395-4191859 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T168093 NZ_CP075632:2087315-2087533 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12309.86 Da
• Isoelectric Point: 4.5392

>AT168093 WP_021547164.1 NZ_CP059003:4191060-4191395 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT168093 NZ_CP075632:2086915-2087289 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: V0TI39