Detailed information of TA system TA168023

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 38423..38677
• Replicon: plasmid pSY3626C1_124k
• Accession: NZ_CP058950
• Organism: Escherichia coli strain SY3626C5

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: HZ988_RS24145
• Protein ID: WP_001351576.1
• Coordinates: 38423..38629 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	38616..38677 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZ988_RS24115	34132..34578	-	447	Protein_42	DUF3363 domain-containing protein	-
HZ988_RS24120	34883..35587	-	705	WP_001067858.1	IS6-like element IS26 family transposase	-
HZ988_RS24125	35651..35776	-	126	Protein_44	antitoxin	-
HZ988_RS24130	36742..37599	-	858	WP_039002220.1	incFII family plasmid replication initiator RepA	-
HZ988_RS24135	37592..37666	-	75	WP_061357229.1	RepA leader peptide Tap	-
HZ988_RS24140	37898..38155	-	258	WP_001541895.1	replication regulatory protein RepA	-
HZ988_RS24145	38423..38629	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	38616..38677	+	62	NuclAT_1	-	Antitoxin
-	38616..38677	+	62	NuclAT_1	-	Antitoxin
-	38616..38677	+	62	NuclAT_1	-	Antitoxin
-	38616..38677	+	62	NuclAT_1	-	Antitoxin
HZ988_RS24150	39019..39231	-	213	WP_001541896.1	ANR family transcriptional regulator	-
HZ988_RS24155	39366..39926	-	561	WP_000139310.1	fertility inhibition protein FinO	-
HZ988_RS24160	39981..40724	-	744	WP_039002216.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(M) / ant(3'')-Ia / aac(3)-IId / lnu(F) / blaTEM-1B / sul3 / aph(3')-Ia / sul2 / floR	-	1..124445	124445

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T168023 WP_001351576.1 NZ_CP058950:c38629-38423 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T168023 NZ_CP075567:c525982-525668 [Pseudomonas fitomaticsae]
ATGATTTTTATTGAGACTCGGATCTTCACCCGTCGAGTAAAAGAGCTGCTGGACGATGACACTTACGCCGCTTTTCAGAA
GCAACTGGTTGTAAGTCCTTCGATTGGCGATGTAATTGAAGGCACAGGCGGGATCCGAAAAACCCGAATCGCAGCGAAAG
GACACGGCAAGCGAGGCGGAGCACGGGTTATCTACTACCACTTCGTTTCGGCATCACAAATCGGGTTATTGATGATCTAC
CCTAAAAATGAACAACACGATCTGTCCAGCGATGAGCGTAAAGCACTCAAGGTCTTGATCGAAAAATGGAGATGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT168023 NZ_CP058950:38616-38677 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin