168019

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3669880..3670607 Replicon chromosome
Accession NZ_CP058948
Organism Escherichia coli strain SY3626C5

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag HZ988_RS17755 Protein ID WP_000550189.1
Coordinates 3670293..3670607 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HZ988_RS17750 Protein ID WP_000560266.1
Coordinates 3669880..3670296 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZ988_RS17740 3665040..3667391 + 2352 WP_000695487.1 alpha-glucosidase -
HZ988_RS17745 3667817..3669835 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
HZ988_RS17750 3669880..3670296 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
HZ988_RS17755 3670293..3670607 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
HZ988_RS17760 3670891..3672027 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
HZ988_RS17765 3672112..3672615 + 504 WP_001333820.1 M48 family metallopeptidase -
HZ988_RS17770 3672692..3673384 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
HZ988_RS17775 3673463..3674449 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T168019 WP_000550189.1 NZ_CP058948:c3670607-3670293 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T168019 NZ_CP075566:1060779-1060991 [Pseudomonas fluorescens]
GTGCAAAGCAGGTTATTGATCAAGGAGCTGGAGGAGGCGGGCTGGACGCTGGATCGGATTACCGGCAGCCATCATCTCTT
CACCCACCGATACAACCCGTACACGATTCCCGTCCCTCACCCGAAAAAGGATTTACCGATCGGGACGGTTAAAAGCATCA
GGAGGCGCGCCGGGCTTTACTGCCCGCCAGCCAGTTACGCAGGAGATCCATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT168019 WP_000560266.1 NZ_CP058948:c3670296-3669880 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT168019 NZ_CP075566:1060991-1061395 [Pseudomonas fluorescens]
ATGCAATATCCAATCTGTATCGAGTGGGGCGACGAGAACACCGCCATCGGTATTCAGATCCCCGATATTCCGGGCGCCGT
AACGGCCGGGGATACGTTTGAAGACGCCTATAACGCGGCGATCGAGATTGCCCACATCATGCTTCAGGAAATAGCGGCTG
ACGGGGAAGCGATTCCTATGCCTTCTTCGGCAGCCGTCCATCGTGGCAACCCGGACTTTGCCGACATGGGATGGGGAATG
CTGGAGCTGGACATCGCGCCGTACATGGGCAAGACCGAGAAGGTCAATGTCACGTTGCCCGGCTATGTGATTCAGCGGAT
TGACCGGTATGTGCGCGAGCACAATGTCAAAAGCCGCTCATCCTTTCTGGCAGATGCGGCGATGGAGAAGCTGGTTCGAC
ACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References