Detailed information of TA system TA167966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2238007..2238647
• Replicon: chromosome
• Accession: NZ_CP058940
• Organism: Klebsiella pneumoniae strain BA39649

Toxin (Protein)

• Gene name: higB
• Uniprot ID: W9BBY1
• Locus tag: HBJ39_RS10810
• Protein ID: WP_016529833.1
• Coordinates: 2238007..2238354 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HBJ39_RS10815
• Protein ID: WP_040181955.1
• Coordinates: 2238354..2238647 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBJ39_RS10800 (2233933)	2233933..2235366	+	1434	WP_002920564.1	glycogen synthase GlgA	-
HBJ39_RS10805 (2235384)	2235384..2237831	+	2448	WP_002920561.1	glycogen phosphorylase	-
HBJ39_RS10810 (2238007)	2238007..2238354	+	348	WP_016529833.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HBJ39_RS10815 (2238354)	2238354..2238647	+	294	WP_040181955.1	helix-turn-helix transcriptional regulator	Antitoxin
HBJ39_RS10820 (2238717)	2238717..2240225	-	1509	WP_023301456.1	glycerol-3-phosphate dehydrogenase	-
HBJ39_RS10825 (2240430)	2240430..2240759	+	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
HBJ39_RS10830 (2240810)	2240810..2241640	+	831	WP_024622927.1	rhomboid family intramembrane serine protease GlpG	-
HBJ39_RS10835 (2241690)	2241690..2242448	+	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (9-87)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13549.56 Da
• Isoelectric Point: 5.6749

>T167966 WP_016529833.1 NZ_CP058940:2238007-2238354 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T167966 NZ_CP075548:810423-810833 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10539.30 Da
• Isoelectric Point: 10.1464

>AT167966 WP_040181955.1 NZ_CP058940:2238354-2238647 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKSLV

Nucleotide

• Length: 294 bp

>AT167966 NZ_CP075548:810176-810442 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: W9BBY1