Detailed information of TA system TA167959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 2150130..2150740
• Replicon: chromosome
• Accession: NZ_CP058908
• Organism: Pseudomonas phenolilytica strain RBPA9

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HU825_RS10405
• Protein ID: WP_165595285.1
• Coordinates: 2150130..2150444 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HU825_RS10410
• Protein ID: WP_165595284.1
• Coordinates: 2150450..2150740 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HU825_RS10385 (HU825_10365)	2146140..2146466	+	327	WP_043298722.1	DUF2523 domain-containing protein	-
HU825_RS10390 (HU825_10370)	2146479..2147657	+	1179	WP_234301984.1	zonular occludens toxin domain-containing protein	-
HU825_RS10395 (HU825_10375)	2147804..2149081	+	1278	WP_234301985.1	hypothetical protein	-
HU825_RS10400 (HU825_10380)	2149081..2150088	+	1008	WP_234301986.1	tyrosine-type recombinase/integrase	-
HU825_RS10405 (HU825_10385)	2150130..2150444	+	315	WP_165595285.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HU825_RS10410 (HU825_10390)	2150450..2150740	+	291	WP_165595284.1	NadS family protein	Antitoxin
HU825_RS10420 (HU825_10400)	2150996..2151688	-	693	WP_156716373.1	pseudouridine synthase	-
HU825_RS10425 (HU825_10405)	2151688..2151894	-	207	WP_156716374.1	cysteine-rich CWC family protein	-
HU825_RS10430 (HU825_10410)	2151956..2153995	-	2040	WP_234301987.1	methionine--tRNA ligase	-
HU825_RS10435 (HU825_10415)	2154178..2155272	+	1095	WP_043295974.1	iron-sulfur cluster carrier protein ApbC	-
HU825_RS10440 (HU825_10420)	2155382..2155591	-	210	WP_003282211.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2146479..2158014	11535

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-86)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11985.77 Da
• Isoelectric Point: 9.8035

>T167959 WP_165595285.1 NZ_CP058908:2150130-2150444 [Pseudomonas phenolilytica]
MIFIETPMFTKRLRELLSDDSYAEFQRQLADRPDMGDVIEGTGGIRKVRVASSGHGKRGGSRVIYYHFTAASQIALLLIY
PKNEKDDLTADERKVLKQIIERWR

Nucleotide

• Length: 315 bp

>T167959 NZ_CP075487:c4276971-4276585 [Escherichia coli]
GTGCTTGCCCCAACTGGGCCATGCAATCATCTTCATGTGATTTGTAATGATCCTGTTTATTACCCCGTTAACGATTGTTA
TTGTGTTTTAGTTGTTAATATTTCTAGTATCAAGGATGGTGTCCCCCACGATCCGTCTTGCGTCTTGAATTCTGGTGATC
ATCGCTTTATCAAGCATCCAAGTTATGTTGTTTACGCTGAAGCTATAATTTGGCGAGTGGATAACATGGTTAGAAAGCAG
CGATCGGGTGAGATTTCCGTTCATGATGATATGCCAGAAGCTACATTCAATAGAATTCTGGACGGTTTTGATATATCTGA
TGAAGTTACGCCAAAGAACCTTAAATTTAAAAATAAATATTGCGTATCATCTATTGATGATGAGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10958.54 Da
• Isoelectric Point: 10.1734

>AT167959 WP_165595284.1 NZ_CP058908:2150450-2150740 [Pseudomonas phenolilytica]
MSRFFEDLLESVQQMDEIRRGERQPSREFTVDALQVKEIRKATGLTQAKFAAMIDVQLGTLRNWEQGRREPTGPAKALLR
AIHNDPKHVIQALSSV

Nucleotide

• Length: 291 bp

>AT167959 NZ_CP075487:c4277510-4277001 [Escherichia coli]
ATGGCATATCTGAAATTGATGAAGCTGCTCTATCTGTCTAACCGCAAGTCGATTTTGAAGCATGGCAGGATGATCGGCGA
AGATAGCCTTTACTCTATGAAATTTGGTCCAGTCATGTCGAATACGCTGAACTTGATTCGTGGTAAGGCTGAAGGTATTG
GTGACTACTGGTACAACCTGATAGAGACGAACGGGCATGATGTGTTGTTGCGTTCAGATCCGAGGGAAATGGATGCAGAC
GAGGTCTTTGATGAGTTGAGCCGTGCAGATATCCGGATTTTAGATGAAATCTATTCTCTGTATGGGCATATGAACCGATT
TGATCTCGCAAACATGACGCATTTAGAAAGCGTTTGTCCAGAGTGGCACAATCCTGGCAATTCTCGTAAGCCTATAGACC
TGAAAGAAATGCTGATCAGTGAGGGTAAAAGCGAGGATGAGGCTAATCGTATAATTGGCAAAATGGAAGAATCTCAGAAA
CTTAAGGAATTTTCTTTGCAATTATCATGA