Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3617774..3618315 Replicon chromosome
Accession NZ_CP058886
Organism Shigella flexneri strain STLEFF_33

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag HZT25_RS17655 Protein ID WP_000615983.1
Coordinates 3618037..3618315 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag HZT25_RS17650 Protein ID WP_000729703.1
Coordinates 3617774..3618034 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT25_RS17630 (3613449) 3613449..3614234 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
HZT25_RS17635 (3614206) 3614206..3615918 + 1713 Protein_3465 flagellar biosynthesis protein FlhA -
HZT25_RS17640 (3616142) 3616142..3616639 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
HZT25_RS17645 (3616815) 3616815..3617564 - 750 WP_000056849.1 C40 family peptidase -
HZT25_RS17650 (3617774) 3617774..3618034 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HZT25_RS17655 (3618037) 3618037..3618315 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HZT25_RS17660 (3618471) 3618471..3619211 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
HZT25_RS17665 (3619182) 3619182..3619949 - 768 WP_000333380.1 class II glutamine amidotransferase -
HZT25_RS17670 (3620155) 3620155..3620733 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T167944 WP_000615983.1 NZ_CP058886:3618037-3618315 [Shigella flexneri]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T167944 NZ_CP075487:691844-692158 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT167944 WP_000729703.1 NZ_CP058886:3617774-3618034 [Shigella flexneri]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT167944 NZ_CP075487:692155-692571 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References