Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 606481..607280 Replicon chromosome
Accession NZ_CP058886
Organism Shigella flexneri strain STLEFF_33

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag HZT25_RS02955 Protein ID WP_000347273.1
Coordinates 606481..606945 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HZT25_RS02960 Protein ID WP_001307405.1
Coordinates 606945..607280 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT25_RS02925 (601482) 601482..601916 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
HZT25_RS02930 (601934) 601934..602812 - 879 WP_001300474.1 PTS N-acetylgalactosamine transporter subunit IID -
HZT25_RS02935 (602802) 602802..603581 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
HZT25_RS02940 (603592) 603592..604065 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HZT25_RS02945 (604088) 604088..605368 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
HZT25_RS02950 (605617) 605617..606426 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HZT25_RS02955 (606481) 606481..606945 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HZT25_RS02960 (606945) 606945..607280 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HZT25_RS02965 (607429) 607429..609000 - 1572 WP_001723936.1 galactarate dehydratase -
HZT25_RS02970 (609375) 609375..610709 + 1335 WP_001723935.1 galactarate/glucarate/glycerate transporter GarP -
HZT25_RS02975 (610725) 610725..611495 + 771 WP_001723934.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T167925 WP_000347273.1 NZ_CP058886:c606945-606481 [Shigella flexneri]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T167925 NZ_CP075484:1856486-1856737 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT167925 WP_001307405.1 NZ_CP058886:c607280-606945 [Shigella flexneri]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT167925 NZ_CP075484:1856734-1856988 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References