Detailed information of TA system TA167914

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 72217..72471
• Replicon: plasmid unnamed1
• Accession: NZ_CP058880
• Organism: Escherichia coli strain STLEFF_29

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: HZT24_RS23925
• Protein ID: WP_001312851.1
• Coordinates: 72217..72366 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	72410..72471 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT24_RS23895 (67464)	67464..68375	-	912	WP_000440183.1	carbamate kinase	-
HZT24_RS23900 (68386)	68386..69606	-	1221	WP_000410951.1	arginine deiminase	-
HZT24_RS23905 (70313)	70313..70927	+	615	Protein_82	VENN motif pre-toxin domain-containing protein	-
HZT24_RS23910 (70927)	70927..71373	-	447	Protein_83	plasmid replication initiator RepA	-
HZT24_RS23915 (71366)	71366..71440	-	75	WP_032336874.1	RepA leader peptide Tap	-
HZT24_RS23920 (71676)	71676..71933	-	258	WP_000083833.1	replication regulatory protein RepA	-
HZT24_RS23925 (72217)	72217..72366	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (72410)	72410..72471	+	62	NuclAT_0	-	Antitoxin
- (72410)	72410..72471	+	62	NuclAT_0	-	Antitoxin
- (72410)	72410..72471	+	62	NuclAT_0	-	Antitoxin
- (72410)	72410..72471	+	62	NuclAT_0	-	Antitoxin
HZT24_RS23930 (72610)	72610..72792	-	183	WP_000968309.1	hypothetical protein	-
HZT24_RS23935 (72893)	72893..73509	+	617	Protein_88	IS1-like element IS1A family transposase	-
HZT24_RS23940 (73547)	73547..75118	-	1572	WP_000381395.1	IS66-like element ISCro1 family transposase	-
HZT24_RS23945 (75138)	75138..75485	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
HZT24_RS23950 (75485)	75485..76162	-	678	WP_001339397.1	IS66-like element accessory protein TnpA	-
HZT24_RS23955 (76217)	76217..76306	+	90	Protein_92	IS1 family transposase	-
HZT24_RS23960 (76607)	76607..76819	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / sul2 / tet(A) / lnu(F) / blaTEM-1B / sul3 / aph(3')-Ia / aac(3)-IId / sitABCD	iutA / iucD / iucC / iucB / iucA	1..121453	121453

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T167914 WP_001312851.1 NZ_CP058880:c72366-72217 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T167914 NZ_CP075480:3609341-3609619 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT167914 NZ_CP058880:72410-72471 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin