Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2372243..2372805 Replicon chromosome
Accession NZ_CP058879
Organism Escherichia coli strain STLEFF_29

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag HZT24_RS11320 Protein ID WP_000605676.1
Coordinates 2372527..2372805 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag HZT24_RS11315 Protein ID WP_000781370.1
Coordinates 2372243..2372527 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT24_RS11300 (2367602) 2367602..2370649 + 3048 WP_012311994.1 formate dehydrogenase-N subunit alpha -
HZT24_RS11305 (2370662) 2370662..2371546 + 885 WP_001240582.1 formate dehydrogenase N subunit beta -
HZT24_RS11310 (2371539) 2371539..2372192 + 654 WP_000045650.1 formate dehydrogenase-N subunit gamma -
HZT24_RS11315 (2372243) 2372243..2372527 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
HZT24_RS11320 (2372527) 2372527..2372805 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HZT24_RS11325 (2372991) 2372991..2374001 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
HZT24_RS11330 (2374135) 2374135..2375832 - 1698 WP_000433464.1 malate dehydrogenase -
HZT24_RS11335 (2375989) 2375989..2376126 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
HZT24_RS11340 (2376228) 2376228..2376443 - 216 WP_000495766.1 biofilm-dependent modulation protein -
HZT24_RS11345 (2376788) 2376788..2377219 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T167896 WP_000605676.1 NZ_CP058879:c2372805-2372527 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T167896 NZ_CP075480:c597976-597512 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGGCATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT167896 WP_000781370.1 NZ_CP058879:c2372527-2372243 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT167896 NZ_CP075480:c598311-597976 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACACTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References