Detailed information of TA system TA167890

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 678457..679256
• Replicon: chromosome
• Accession: NZ_CP058879
• Organism: Escherichia coli strain STLEFF_29

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: HZT24_RS03300
• Protein ID: WP_000347273.1
• Coordinates: 678457..678921 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: HZT24_RS03305
• Protein ID: WP_001308975.1
• Coordinates: 678921..679256 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT24_RS03270 (673458)	673458..673892	-	435	WP_000948834.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
HZT24_RS03275 (673910)	673910..674788	-	879	WP_001298314.1	PTS N-acetylgalactosamine transporter subunit IID	-
HZT24_RS03280 (674778)	674778..675557	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
HZT24_RS03285 (675568)	675568..676041	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
HZT24_RS03290 (676064)	676064..677344	-	1281	WP_000681961.1	tagatose-bisphosphate aldolase subunit KbaZ	-
HZT24_RS03295 (677593)	677593..678402	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
HZT24_RS03300 (678457)	678457..678921	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
HZT24_RS03305 (678921)	678921..679256	-	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
HZT24_RS03310 (679405)	679405..680976	-	1572	WP_001273756.1	galactarate dehydratase	-
HZT24_RS03315 (681351)	681351..682685	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
HZT24_RS03320 (682701)	682701..683471	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T167890 WP_000347273.1 NZ_CP058879:c678921-678457 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T167890 NZ_CP075477:3661509-3661787 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT167890 WP_001308975.1 NZ_CP058879:c679256-678921 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT167890 NZ_CP075477:3661246-3661506 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08