Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 66128..66381 Replicon plasmid unnamed3
Accession NZ_CP058877
Organism Escherichia coli strain STLEFF_47

Toxin (Protein)


Gene name srnB Uniprot ID G9G1E3
Locus tag HZT32_RS25970 Protein ID WP_001312851.1
Coordinates 66232..66381 (+) Length 50 a.a.

Antitoxin (RNA)


Gene name srnC
Locus tag -
Coordinates 66128..66187 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT32_RS25945 (62855) 62855..63601 + 747 WP_000205770.1 conjugal transfer pilus acetylase TraX -
HZT32_RS25950 (63656) 63656..64216 + 561 WP_063097573.1 fertility inhibition protein FinO -
HZT32_RS25955 (64348) 64348..64548 + 201 WP_015059022.1 hypothetical protein -
HZT32_RS25960 (64934) 64934..65533 + 600 WP_032083981.1 PIN domain-containing protein -
HZT32_RS25965 (65595) 65595..65927 + 333 WP_152916585.1 hypothetical protein -
- (66128) 66128..66187 - 60 NuclAT_1 - Antitoxin
- (66128) 66128..66187 - 60 NuclAT_1 - Antitoxin
- (66128) 66128..66187 - 60 NuclAT_1 - Antitoxin
- (66128) 66128..66187 - 60 NuclAT_1 - Antitoxin
HZT32_RS25970 (66232) 66232..66381 + 150 WP_001312851.1 Hok/Gef family protein Toxin
HZT32_RS25975 (66665) 66665..66913 + 249 WP_000083837.1 replication regulatory protein RepA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-55 / blaTEM-1B / rmtB - 1..67224 67224


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(4-45)


Sequences

Toxin        


Download         Length: 50 a.a.        Molecular weight: 5542.67 Da        Isoelectric Point: 8.7678

>T167886 WP_001312851.1 NZ_CP058877:66232-66381 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Download         Length: 150 bp

>T167886 NZ_CP075477:2692891-2692998 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 60 bp

>AT167886 NZ_CP058877:c66187-66128 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829C8K5

Antitoxin

Download structure file

References