Detailed information of TA system TA167883

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 26650..26919
• Replicon: plasmid unnamed3
• Accession: NZ_CP058877
• Organism: Escherichia coli strain STLEFF_47

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: HZT32_RS25740
• Protein ID: WP_001372321.1
• Coordinates: 26794..26919 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	26650..26715 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT32_RS25710	22360..22887	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
HZT32_RS25715	22945..23178	+	234	WP_000006003.1	DUF905 family protein	-
HZT32_RS25720	23239..25262	+	2024	Protein_30	ParB/RepB/Spo0J family partition protein	-
HZT32_RS25725	25331..25765	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HZT32_RS25730	25762..26524	+	763	Protein_32	plasmid SOS inhibition protein A	-
-	26493..26717	+	225	NuclAT_0	-	-
-	26493..26717	+	225	NuclAT_0	-	-
-	26493..26717	+	225	NuclAT_0	-	-
-	26493..26717	+	225	NuclAT_0	-	-
-	26650..26715	-	66	-	-	Antitoxin
HZT32_RS25735	26703..26852	+	150	Protein_33	plasmid maintenance protein Mok	-
HZT32_RS25740	26794..26919	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
HZT32_RS25745	27238..27534	-	297	Protein_35	hypothetical protein	-
HZT32_RS25750	27834..28130	+	297	WP_001272251.1	hypothetical protein	-
HZT32_RS25755	28241..29062	+	822	WP_001234445.1	DUF932 domain-containing protein	-
HZT32_RS25760	29359..30006	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
HZT32_RS25765	30283..30666	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
HZT32_RS25770	30857..31543	+	687	WP_015059009.1	PAS domain-containing protein	-
HZT32_RS25775	31637..31864	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55 / blaTEM-1B / rmtB	-	1..67224	67224

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T167883 WP_001372321.1 NZ_CP058877:26794-26919 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T167883 NZ_CP075477:c2487341-2487165 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT167883 NZ_CP058877:c26715-26650 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin