Detailed information of TA system TA167875

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 137894..138148
• Replicon: plasmid unnamed1
• Accession: NZ_CP058875
• Organism: Escherichia coli strain STLEFF_47

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: HZT32_RS24805
• Protein ID: WP_001312851.1
• Coordinates: 137999..138148 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	137894..137955 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT32_RS24785 (135609)	135609..136355	+	747	WP_039005755.1	conjugal transfer pilus acetylase TraX	-
HZT32_RS24790 (136410)	136410..136970	+	561	WP_039005753.1	fertility inhibition protein FinO	-
HZT32_RS24795 (137106)	137106..137318	+	213	WP_001299730.1	ANR family transcriptional regulator	-
HZT32_RS24800 (137564)	137564..137638	+	75	Protein_141	endonuclease	-
- (137894)	137894..137955	-	62	NuclAT_1	-	Antitoxin
- (137894)	137894..137955	-	62	NuclAT_1	-	Antitoxin
- (137894)	137894..137955	-	62	NuclAT_1	-	Antitoxin
- (137894)	137894..137955	-	62	NuclAT_1	-	Antitoxin
HZT32_RS24805 (137999)	137999..138148	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
HZT32_RS24810 (138432)	138432..138689	+	258	WP_000083845.1	replication regulatory protein RepA	-
HZT32_RS24815 (138706)	138706..138957	-	252	WP_223195197.1	replication protein RepA	-
HZT32_RS24820 (138948)	138948..138995	+	48	WP_229471593.1	hypothetical protein	-
HZT32_RS24825 (138988)	138988..139470	+	483	WP_001273588.1	hypothetical protein	-
HZT32_RS24830 (139463)	139463..140320	+	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
HZT32_RS24835 (141283)	141283..141534	+	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
HZT32_RS24840 (141531)	141531..141818	+	288	WP_000222766.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / iucA / iucB / iucC / iucD / iutA / vat	1..152887	152887

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T167875 WP_001312851.1 NZ_CP058875:137999-138148 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T167875 NZ_CP075477:718629-719006 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTATGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT167875 NZ_CP058875:c137955-137894 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin