Detailed information of TA system TA167868

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4758900..4759502
• Replicon: chromosome
• Accession: NZ_CP058874
• Organism: Escherichia coli strain STLEFF_47

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HZT32_RS23050
• Protein ID: WP_000897305.1
• Coordinates: 4759191..4759502 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HZT32_RS23045
• Protein ID: WP_000356397.1
• Coordinates: 4758900..4759190 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT32_RS23025 (4755402)	4755402..4756304	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HZT32_RS23030 (4756301)	4756301..4756936	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HZT32_RS23035 (4756933)	4756933..4757862	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
HZT32_RS23040 (4758077)	4758077..4758295	-	219	WP_001295676.1	CopG family transcriptional regulator	-
HZT32_RS23045 (4758900)	4758900..4759190	-	291	WP_000356397.1	NadS family protein	Antitoxin
HZT32_RS23050 (4759191)	4759191..4759502	-	312	WP_000897305.1	hypothetical protein	Toxin
HZT32_RS23055 (4759731)	4759731..4760639	+	909	WP_001318161.1	alpha/beta hydrolase	-
HZT32_RS23060 (4760703)	4760703..4761644	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HZT32_RS23065 (4761689)	4761689..4762126	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
HZT32_RS23070 (4762123)	4762123..4762995	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HZT32_RS23075 (4762989)	4762989..4763588	-	600	WP_001315111.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T167868 WP_000897305.1 NZ_CP058874:c4759502-4759191 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T167868 NZ_CP075474:c3613895-3613497 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT167868 WP_000356397.1 NZ_CP058874:c4759190-4758900 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT167868 NZ_CP075474:c3614191-3613898 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7