Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3549882..3550561 Replicon chromosome
Accession NZ_CP058874
Organism Escherichia coli strain STLEFF_47

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag HZT32_RS17355 Protein ID WP_000057523.1
Coordinates 3550259..3550561 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag HZT32_RS17350 Protein ID WP_000806442.1
Coordinates 3549882..3550223 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT32_RS17340 (3546125) 3546125..3547057 - 933 WP_000883024.1 glutaminase A -
HZT32_RS17345 (3547320) 3547320..3549824 + 2505 WP_000083999.1 copper-exporting P-type ATPase CopA -
HZT32_RS17350 (3549882) 3549882..3550223 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
HZT32_RS17355 (3550259) 3550259..3550561 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HZT32_RS17360 (3550694) 3550694..3551488 + 795 WP_000365161.1 TraB/GumN family protein -
HZT32_RS17365 (3551692) 3551692..3552171 + 480 WP_000186638.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
HZT32_RS17370 (3552259) 3552259..3553860 - 1602 WP_063268717.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
HZT32_RS17375 (3554078) 3554078..3555298 + 1221 WP_001251634.1 fosmidomycin MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T167864 WP_000057523.1 NZ_CP058874:c3550561-3550259 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T167864 NZ_CP075474:2596315-2596422 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCATCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT167864 WP_000806442.1 NZ_CP058874:c3550223-3549882 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT167864 NZ_CP075474:c2596262-2596201 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References