Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 689474..690273 Replicon chromosome
Accession NZ_CP058874
Organism Escherichia coli strain STLEFF_47

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag HZT32_RS03400 Protein ID WP_000347251.1
Coordinates 689474..689938 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag HZT32_RS03405 Protein ID WP_001296435.1
Coordinates 689938..690273 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT32_RS03370 (684475) 684475..684909 - 435 WP_000948823.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
HZT32_RS03375 (684927) 684927..685805 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
HZT32_RS03380 (685795) 685795..686574 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
HZT32_RS03385 (686585) 686585..687058 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HZT32_RS03390 (687081) 687081..688361 - 1281 WP_000681938.1 tagatose-bisphosphate aldolase subunit KbaZ -
HZT32_RS03395 (688610) 688610..689419 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HZT32_RS03400 (689474) 689474..689938 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HZT32_RS03405 (689938) 689938..690273 - 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HZT32_RS03410 (690422) 690422..691993 - 1572 WP_001273941.1 galactarate dehydratase -
HZT32_RS03415 (692368) 692368..693702 + 1335 WP_000979025.1 galactarate/glucarate/glycerate transporter GarP -
HZT32_RS03420 (693718) 693718..694488 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T167849 WP_000347251.1 NZ_CP058874:c689938-689474 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T167849 NZ_CP075472:c3943548-3943261 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT167849 WP_001296435.1 NZ_CP058874:c690273-689938 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT167849 NZ_CP075472:c3943274-3942972 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References