Detailed information of TA system TA167797

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 119701..120428
• Replicon: plasmid unnamed1
• Accession: NZ_CP058860
• Organism: Klebsiella quasipneumoniae strain STLEFF_28

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: HZT23_RS26025
• Protein ID: WP_011251285.1
• Coordinates: 120117..120428 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HZT23_RS26020
• Protein ID: WP_011251286.1
• Coordinates: 119701..120120 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT23_RS25995 (HZT23_25865)	115293..116660	+	1368	WP_017900870.1	formimidoylglutamate deiminase	-
HZT23_RS26000 (HZT23_25870)	117185..117720	+	536	Protein_123	transposase	-
HZT23_RS26005 (HZT23_25875)	117834..118100	-	267	WP_017900868.1	hypothetical protein	-
HZT23_RS26010 (HZT23_25880)	118204..119172	+	969	WP_074168518.1	IS5-like element IS903B family transposase	-
HZT23_RS26015 (HZT23_25885)	119218..119631	-	414	WP_017901337.1	hypothetical protein	-
HZT23_RS26020 (HZT23_25890)	119701..120120	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
HZT23_RS26025 (HZT23_25895)	120117..120428	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
HZT23_RS26030 (HZT23_25900)	120633..121070	-	438	Protein_129	DDE-type integrase/transposase/recombinase	-
HZT23_RS26035 (HZT23_25905)	121184..121561	+	378	WP_004118218.1	transposase	-
HZT23_RS26040 (HZT23_25910)	121558..121905	+	348	WP_032430752.1	IS66 family insertion sequence element accessory protein TnpB	-
HZT23_RS26045 (HZT23_25915)	121954..123492	+	1539	WP_017901237.1	IS66 family transposase	-
HZT23_RS26050 (HZT23_25920)	123532..123711	+	180	Protein_133	IS5/IS1182 family transposase	-
HZT23_RS26055 (HZT23_25925)	124257..125237	-	981	WP_012569499.1	IS5-like element ISKpn26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	catA1 / tet(D)	-	1..204335	204335
	-	inside	IScluster/Tn	-	-	117592..125237	7645

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T167797 WP_011251285.1 NZ_CP058860:c120428-120117 [Klebsiella quasipneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T167797 NZ_CP075465:983406-983741 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT167797 WP_011251286.1 NZ_CP058860:c120120-119701 [Klebsiella quasipneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT167797 NZ_CP075465:983158-983406 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGACTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1