Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3828441..3829240 Replicon chromosome
Accession NZ_CP058853
Organism Shigella flexneri strain STLEFF_38

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag HZT29_RS18495 Protein ID WP_000347251.1
Coordinates 3828776..3829240 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HZT29_RS18490 Protein ID WP_001307405.1
Coordinates 3828441..3828776 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT29_RS18475 (3824226) 3824226..3824996 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
HZT29_RS18480 (3825012) 3825012..3826346 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
HZT29_RS18485 (3826721) 3826721..3828292 + 1572 WP_001273753.1 galactarate dehydratase -
HZT29_RS18490 (3828441) 3828441..3828776 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HZT29_RS18495 (3828776) 3828776..3829240 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HZT29_RS18500 (3829295) 3829295..3830104 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HZT29_RS18505 (3830353) 3830353..3831633 + 1281 WP_000681905.1 tagatose-bisphosphate aldolase subunit KbaZ -
HZT29_RS18510 (3831656) 3831656..3832129 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HZT29_RS18515 (3832140) 3832140..3832919 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
HZT29_RS18520 (3832909) 3832909..3833787 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
HZT29_RS18525 (3833805) 3833805..3834239 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3817671..3829240 11569


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T167757 WP_000347251.1 NZ_CP058853:3828776-3829240 [Shigella flexneri]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T167757 NZ_CP075452:1879258-1879360 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT167757 WP_001307405.1 NZ_CP058853:3828441-3828776 [Shigella flexneri]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT167757 NZ_CP075452:c1879367-1879222 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References