Detailed information of TA system TA167756

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3779776..3780503
• Replicon: chromosome
• Accession: NZ_CP058853
• Organism: Shigella flexneri strain STLEFF_38

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HZT29_RS18250
• Protein ID: WP_240764965.1
• Coordinates: 3780189..3780503 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HZT29_RS18245
• Protein ID: WP_000560255.1
• Coordinates: 3779776..3780192 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT29_RS18235 (3775036)	3775036..3777387	+	2352	WP_000695495.1	alpha-glucosidase	-
HZT29_RS18240 (3777713)	3777713..3779731	+	2019	WP_047632951.1	NADPH-dependent 2,4-dienoyl-CoA reductase	-
HZT29_RS18245 (3779776)	3779776..3780192	-	417	WP_000560255.1	type II toxin-antitoxin system antitoxin HigA	Antitoxin
HZT29_RS18250 (3780189)	3780189..3780503	-	315	WP_240764965.1	type II toxin-antitoxin system toxin HigB	Toxin
HZT29_RS18255 (3780788)	3780788..3781924	-	1137	WP_000018690.1	23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG	-
HZT29_RS18260 (3782009)	3782009..3782512	+	504	WP_001295542.1	M48 family metallopeptidase	-
HZT29_RS18265 (3782589)	3782589..3783272	+	684	WP_001183041.1	vancomycin high temperature exclusion protein	-
HZT29_RS18270 (3783351)	3783351..3784337	+	987	WP_053274491.1	Gfo/Idh/MocA family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-92)

Antitoxin

• Antitoxin HigA (1-138)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12131.16 Da
• Isoelectric Point: 10.0409

>T167756 WP_240764965.1 NZ_CP058853:c3780503-3780189 [Shigella flexneri]
MHLITQKALKDVAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Nucleotide

• Length: 315 bp

>T167756 NZ_CP075452:810423-810833 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 14951.41 Da
• Isoelectric Point: 4.5577

>AT167756 WP_000560255.1 NZ_CP058853:c3780192-3779776 [Shigella flexneri]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNAPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Nucleotide

• Length: 417 bp

>AT167756 NZ_CP075452:810176-810442 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA