Detailed information of TA system TA167755

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3718425..3719118
• Replicon: chromosome
• Accession: NZ_CP058853
• Organism: Shigella flexneri strain STLEFF_38

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: HZT29_RS17950
• Protein ID: WP_000415584.1
• Coordinates: 3718822..3719118 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: HZT29_RS17945
• Protein ID: WP_000650107.1
• Coordinates: 3718425..3718820 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT29_RS17935 (3714289)	3714289..3716547	-	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-
HZT29_RS17940 (3716685)	3716685..3718292	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
HZT29_RS17945 (3718425)	3718425..3718820	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
HZT29_RS17950 (3718822)	3718822..3719118	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
HZT29_RS17955 (3719323)	3719323..3719805	-	483	WP_000183505.1	GyrI-like domain-containing protein	-
HZT29_RS17960 (3719858)	3719858..3720250	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
HZT29_RS17965 (3720402)	3720402..3721061	+	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
HZT29_RS17970 (3721058)	3721058..3722407	+	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
HZT29_RS17975 (3722453)	3722453..3722785	-	333	WP_000917684.1	DUF2645 family protein	-
HZT29_RS17980 (3723104)	3723104..3723685	+	582	WP_001569186.1	NADPH:quinone oxidoreductase MdaB	-
HZT29_RS17985 (3723716)	3723716..3724030	+	315	WP_000958598.1	putative quinol monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T167755 WP_000415584.1 NZ_CP058853:c3719118-3718822 [Shigella flexneri]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T167755 NZ_CP075452:732262-732747 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT167755 WP_000650107.1 NZ_CP058853:c3718820-3718425 [Shigella flexneri]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT167755 NZ_CP075452:731972-732265 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65