Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 649806..650533 Replicon chromosome
Accession NZ_CP058849
Organism Shigella flexneri strain STLEFF_36

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag HZT28_RS03185 Protein ID WP_000550189.1
Coordinates 649806..650120 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HZT28_RS03190 Protein ID WP_000560266.1
Coordinates 650117..650533 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT28_RS03165 (645973) 645973..646959 - 987 WP_023281494.1 Gfo/Idh/MocA family oxidoreductase -
HZT28_RS03170 (647038) 647038..647721 - 684 WP_001183042.1 vancomycin high temperature exclusion protein -
HZT28_RS03175 (647798) 647798..648301 - 504 WP_001295542.1 M48 family metallopeptidase -
HZT28_RS03180 (648386) 648386..649522 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
HZT28_RS03185 (649806) 649806..650120 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
HZT28_RS03190 (650117) 650117..650533 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
HZT28_RS03195 (650578) 650578..652596 - 2019 WP_049253702.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
HZT28_RS03200 (653022) 653022..655373 - 2352 WP_024238710.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T167708 WP_000550189.1 NZ_CP058849:649806-650120 [Shigella flexneri]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T167708 NZ_CP075354:1065-1442 [Pseudochrobactrum algeriensis]
ATGAATGACGACGAGCGCCCGCTAGAATGGATCGGTAGCAGCTACAAAGACCTGATGCAGCTTCCTGCCGATGTGCGGAA
GTTTTTCGGTTTTGCGCTTAGTTTCGCGCAGCAGGGCGAGCAGCACGCCGCCGCAAAAGTCCTCAAAGGATTTGGCGGCG
CTGGTGTCCTCGAACTGGTCGAGAACGACGCTGGCGGGACATATCGGGCCGTGTACACGGTCAAATTCGAGGAAGCTGTT
TTCGTGCTGCATTGCTTTCAGAAGAAGAGCAAGAGCGGCATCGCCACACCGAAAGCGGACATGGACGTTATCCGCGCCCG
GTTGAAGGTGGCGGAGATAGTGGCGAAGGAGATGAGACATGAGAACCCGCATCGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT167708 WP_000560266.1 NZ_CP058849:650117-650533 [Shigella flexneri]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT167708 NZ_CP075354:1423-1758 [Pseudochrobactrum algeriensis]
ATGAGAACCCGCATCGTTAATGGGGTCGAGGTCACGACGAGTTCCGGCAATGTCTTTGCCGATCTCGGCCTACCCGATGC
CGAAAAGCTGAAAATGAAATCCGGTCTGATGATCGAGATCACTAAGGCCGTGCGTCGGCTCGACCTTACACAAGAGGAAG
CCGGGCGGCGCATGGGACTTCCGCAGCCGAAAGTGTCGGCGCTCCTACGGGGCGACTTCACAAACCTCTCGGAACGGAAG
CTGATGGAATGTCTGAACCGGCTTGGTTACGACATCGAAATTACAGTGAAGCCGGCCGCCGATGCTGTCGGGCATATGAC
GCTCGCGGTAGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References