Detailed information of TA system TA167705

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 40392..40661
• Replicon: plasmid unnamed2
• Accession: NZ_CP058848
• Organism: Shigella flexneri strain STLEFF_35

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: HZT27_RS23900
• Protein ID: WP_001372321.1
• Coordinates: 40536..40661 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	40392..40457 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT27_RS23850 (35893)	35893..36243	+	351	WP_000493380.1	hypothetical protein	-
HZT27_RS23855 (36313)	36313..36921	+	609	WP_000715519.1	hypothetical protein	-
HZT27_RS23860 (36989)	36989..37771	+	783	WP_001372173.1	site-specific integrase	-
HZT27_RS23865 (37909)	37909..38184	-	276	WP_000239529.1	hypothetical protein	-
HZT27_RS23870 (38178)	38178..38822	-	645	WP_000633913.1	division plane positioning ATPase MipZ	-
HZT27_RS23875 (39051)	39051..39329	+	279	Protein_46	plasmid segregation protein ParM	-
HZT27_RS23880 (39325)	39325..39507	+	183	Protein_47	conjugation system SOS inhibitor PsiB family protein	-
HZT27_RS23885 (39504)	39504..40266	+	763	Protein_48	plasmid SOS inhibition protein A	-
HZT27_RS23890 (40235)	40235..40423	-	189	WP_001299721.1	hypothetical protein	-
- (40392)	40392..40457	+	66	NuclAT_1	-	-
- (40392)	40392..40457	-	66	NuclAT_0	-	Antitoxin
- (40392)	40392..40457	-	66	NuclAT_0	-	Antitoxin
- (40235)	40235..40459	+	225	NuclAT_0	-	-
- (40235)	40235..40459	+	225	NuclAT_0	-	-
- (40235)	40235..40459	+	225	NuclAT_0	-	-
- (40235)	40235..40459	+	225	NuclAT_0	-	-
- (40235)	40235..40459	-	225	NuclAT_0	-	-
HZT27_RS23895 (40445)	40445..40594	+	150	Protein_50	plasmid maintenance protein Mok	-
HZT27_RS23900 (40536)	40536..40661	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
HZT27_RS23905 (41056)	41056..42723	-	1668	WP_012372796.1	group II intron reverse transcriptase/maturase	-
HZT27_RS23910 (43452)	43452..43625	-	174	Protein_53	hypothetical protein	-
HZT27_RS23915 (43695)	43695..43901	+	207	WP_240767151.1	single-stranded DNA-binding protein	-
HZT27_RS23920 (43926)	43926..44213	+	288	WP_000107535.1	hypothetical protein	-
HZT27_RS23925 (44331)	44331..45152	+	822	WP_073520018.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T167705 WP_001372321.1 NZ_CP058848:40536-40661 [Shigella flexneri]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T167705 NZ_CP075345:c3798228-3797860 [Enterobacter kobei]
ATGAAAAATCTACCTGCTACAATTTCGCGGGCGGCGAAGCCCTGCCTGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTCTGCTTGAACAGCACTATGGTCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTAATTAAGGAACATATTG
ATGCTGGGATTATCCTGGCCGATGCAGTCAACTTCCTGGTAGATAAGTACGAACTGGTTCGCATTGATCGCAGAGGATTT
AGCTCACAGGGACAAGTACCATATTTGACCGTGACAGATATCCTTCATGCCAGAAGAGCATGTGGATTAATGAAAAGTTG
CTCTTATCGGGAAGTAAGTAATATTGTTCTTAGTCATTCGCGGCAGTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT167705 NZ_CP058848:c40457-40392 [Shigella flexneri]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin