Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3800592..3801319 Replicon chromosome
Accession NZ_CP058846
Organism Shigella flexneri strain STLEFF_35

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag HZT27_RS18665 Protein ID WP_000550189.1
Coordinates 3801005..3801319 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HZT27_RS18660 Protein ID WP_000560266.1
Coordinates 3800592..3801008 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT27_RS18650 (3795752) 3795752..3798103 + 2352 WP_000695502.1 alpha-glucosidase -
HZT27_RS18655 (3798529) 3798529..3800547 + 2019 WP_000121449.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
HZT27_RS18660 (3800592) 3800592..3801008 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
HZT27_RS18665 (3801005) 3801005..3801319 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
HZT27_RS18670 (3801603) 3801603..3802739 - 1137 WP_240767007.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
HZT27_RS18675 (3802824) 3802824..3803327 + 504 WP_001300832.1 M48 family metallopeptidase -
HZT27_RS18680 (3803404) 3803404..3804096 + 693 WP_000942546.1 vancomycin high temperature exclusion protein -
HZT27_RS18685 (3804175) 3804175..3805161 + 987 WP_001301393.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T167703 WP_000550189.1 NZ_CP058846:c3801319-3801005 [Shigella flexneri]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T167703 NZ_CP075345:3527500-3527862 [Enterobacter kobei]
ATGTGGAATGTCGAGACAACGGACAGATTTGATCAATGGTACTTCGAACAAACAACAGCATTAAAAGAAGATGTGCTAGC
GATGATGCATATTCTTGCTGAGTTTGGCCCAACGCTTGGGCGGCCATACGTAGATACCGTGAAGGCTTCAGCCTATGCGA
ACATGAAGGAATTACGGATTCAACATGCGGGAAATCCCATCAGAGCTTTTTTTGCTTTTGATCCTGACCGTAAGGCAATT
GTGCTCTGTGCTGGAAACAAAACCGGTTGCAATCAAAAACGGTTTTATAACGAAATGATTACGCTGGCTGACGTTGAGTA
CAGCAGACATCTGGCGGATAAGGAGGCCATATGGCAACGCTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT167703 WP_000560266.1 NZ_CP058846:c3801008-3800592 [Shigella flexneri]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT167703 NZ_CP075345:3527850-3528149 [Enterobacter kobei]
ATGGCAACGCTAAAAGAACTCATGGCACAACAGAGCGCGGAAAGTCAGGAGCGTATTGCGGCTAAAGTTGATGTAATACG
CAGAATTGTTGCCCTGAACCAACTGCGGGAAGAGCTCAATATTTCGCAGACAGAACTGGCCGCGGCAATGGGGGTAAAAC
AACCAACTGTGGCAAAAATCGAACAGCCGGGAAACGATCCCCGGCTTTCAACACTCAAACGCTATGTCAGTGCGCTCGGC
GGTGAAATGAGCATTGATGTGACGTTACCGAATGGGAAACGAATAGCGTTTCAGATTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References