Detailed information of TA system TA167681

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 793410..794104
• Replicon: chromosome
• Accession: NZ_CP058846
• Organism: Shigella flexneri strain STLEFF_35

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: HZT27_RS03735
• Protein ID: WP_001263493.1
• Coordinates: 793706..794104 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: HZT27_RS03730
• Protein ID: WP_000554757.1
• Coordinates: 793410..793703 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT27_RS03710 (789050)	789050..789547	+	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-
HZT27_RS03715 (789763)	789763..791475	-	1713	Protein_718	flagellar biosynthesis protein FlhA	-
HZT27_RS03720 (791447)	791447..792232	+	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
HZT27_RS03725 (792303)	792303..793358	+	1056	WP_001226164.1	DNA polymerase IV	-
HZT27_RS03730 (793410)	793410..793703	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HZT27_RS03735 (793706)	793706..794104	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HZT27_RS03740 (794114)	794114..794566	+	453	WP_097749298.1	GNAT family N-acetyltransferase	-
HZT27_RS03745 (794812)	794812..795015	+	204	Protein_724	RtcB family protein	-
HZT27_RS03750 (795014)	795014..795535	+	522	Protein_725	peptide chain release factor H	-
HZT27_RS03755 (795592)	795592..797049	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
HZT27_RS03760 (797310)	797310..797768	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (798364)	798364..798444	+	81	NuclAT_12	-	-
- (798364)	798364..798444	+	81	NuclAT_12	-	-
- (798364)	798364..798444	+	81	NuclAT_12	-	-
- (798364)	798364..798444	+	81	NuclAT_12	-	-
HZT27_RS03765 (797860)	797860..799104	+	1245	WP_000189541.1	esterase FrsA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T167681 WP_001263493.1 NZ_CP058846:793706-794104 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T167681 NZ_CP075327:c21489-21157 [Xanthomonas citri pv. citri]
ATGGATCGCGGCGATGTCTATTTGGTTTCGCTCGATCCCACGTCCGGCCATGAACAACAGGGCACGCGCCCGGTGCTGAT
TGTGTCGCCAAGCGCGTTCAACCGCCTGACGAAAACACCGGTCGTGCTGCCGATCACCAGTGGGGGTAACTTCGCCCGCA
CGGCAGGCTTTACCGTGTCGCTCATGGGTGCGGGTACCAACACAACTGGCGTAGTTCGCTGCGATCAGCCCCGCGCTCTC
GATCTAGCCTCGCGGCGCGCGCGCAAGCTGGAAACCGTGCCGTCCCCGATCATGGATGAGGTATTAGCGAAGCTGAGCGC
GATCCTTGAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT167681 WP_000554757.1 NZ_CP058846:793410-793703 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT167681 NZ_CP075327:c21746-21489 [Xanthomonas citri pv. citri]
ATGCATACGACCAATCTGCGTAAGGTCGGTGGCTCGATCATGTTGGCTGTCCCGCCCGCCATTCTGGATATGCTGCGTCT
GCGTGCCGGCGCTACCGTTGGCTTGGCCGTCGATCATGGTCGCCTGGTGATCGAACCCACGCTGCGTCCGTACTACAGCC
TGGATGAGCTGCTGGCCCAATGCGACGCATCCGCCGAACTCTCCACCGAAGATCGGGAATGGCTCGACGCCAAGCCTGTT
GGCAGCGAGCTGCTGTGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6