Detailed information of TA system TA167672

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4083250..4083944
• Replicon: chromosome
• Accession: NZ_CP058839
• Organism: Shigella flexneri strain STLEFF_34

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: HZT26_RS19880
• Protein ID: WP_001263489.1
• Coordinates: 4083546..4083944 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: HZT26_RS19875
• Protein ID: WP_000554758.1
• Coordinates: 4083250..4083543 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT26_RS19855 (4078882)	4078882..4079379	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
HZT26_RS19860 (4079603)	4079603..4081315	-	1713	Protein_3884	flagellar biosynthesis protein FlhA	-
HZT26_RS19865 (4081287)	4081287..4082072	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
HZT26_RS19870 (4082143)	4082143..4083198	+	1056	WP_001226164.1	DNA polymerase IV	-
HZT26_RS19875 (4083250)	4083250..4083543	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HZT26_RS19880 (4083546)	4083546..4083944	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HZT26_RS19885 (4083954)	4083954..4084406	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
HZT26_RS19890 (4084724)	4084724..4084930	+	207	Protein_3890	RtcB family protein	-
HZT26_RS19895 (4084926)	4084926..4085447	+	522	Protein_3891	peptide chain release factor H	-
HZT26_RS19900 (4085504)	4085504..4086961	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
HZT26_RS19905 (4087222)	4087222..4087680	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (4088276)	4088276..4088356	+	81	NuclAT_12	-	-
- (4088276)	4088276..4088356	+	81	NuclAT_12	-	-
- (4088276)	4088276..4088356	+	81	NuclAT_12	-	-
- (4088276)	4088276..4088356	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T167672 WP_001263489.1 NZ_CP058839:4083546-4083944 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T167672 NZ_CP075324:2861-3217 [Acinetobacter sp. BHS4]
ATGTGGACAGTCATTACAACGGATCTGTTTAATGAATGGCTTGAACAGCAAGATGAAGCAACACAAGAGAAAGTCTTAGC
TGCATTGGTTGTTCTACAACAGCAAGGGCCTAGTTTAGGTCGTCCTTTAGTAGATACCGTCTACGATTCTAAATTTACCA
ATATGAAGGAACTACGTGTTCAGCATCGTGGTAAACCGTTAAGGGCATTTTTTGCATTCGATCCCCTAAGACAAGCGATT
GTGCTGTGTATTGGTGATAAGAGTAATAAAAAGCGTTTTTATACAGAGATGCTAGCAATTGCAGATGAGCAATACGCACT
TCATCTCTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT167672 WP_000554758.1 NZ_CP058839:4083250-4083543 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT167672 NZ_CP075324:3210-3512 [Acinetobacter sp. BHS4]
ATGGCTAAGAGCTTACAAGAATTATTGGCTAGTCGCTCTCCAGAGAGTCAAGCTCGTATTCAAAAAATGGCTGATGAATT
ACTTTTAGAAAGTCAGCTTCACCTTATCCGAGAAGAGTTAGAAATTTCCCAGAAAGAACTAGCTGCAACCCTTGGAATTA
AACAGCCGTCATTATCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTTGAAGCAATG
GGTGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGGTTCAATGTCTAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3