Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2398324..2399123 Replicon chromosome
Accession NZ_CP058839
Organism Shigella flexneri strain STLEFF_34

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag HZT26_RS11745 Protein ID WP_000347273.1
Coordinates 2398659..2399123 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HZT26_RS11740 Protein ID WP_001307405.1
Coordinates 2398324..2398659 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT26_RS11725 (2394109) 2394109..2394879 - 771 WP_001723934.1 2-dehydro-3-deoxyglucarate aldolase -
HZT26_RS11730 (2394895) 2394895..2396229 - 1335 WP_001723935.1 galactarate/glucarate/glycerate transporter GarP -
HZT26_RS11735 (2396604) 2396604..2398175 + 1572 WP_001723936.1 galactarate dehydratase -
HZT26_RS11740 (2398324) 2398324..2398659 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HZT26_RS11745 (2398659) 2398659..2399123 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HZT26_RS11750 (2399178) 2399178..2399987 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HZT26_RS11755 (2400236) 2400236..2401516 + 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
HZT26_RS11760 (2401539) 2401539..2402012 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HZT26_RS11765 (2402023) 2402023..2402802 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
HZT26_RS11770 (2402792) 2402792..2403670 + 879 WP_001300474.1 PTS N-acetylgalactosamine transporter subunit IID -
HZT26_RS11775 (2403688) 2403688..2404122 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2389177..2399123 9946


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T167668 WP_000347273.1 NZ_CP058839:2398659-2399123 [Shigella flexneri]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T167668 NZ_CP075320:2574-2888 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT167668 WP_001307405.1 NZ_CP058839:2398324-2398659 [Shigella flexneri]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT167668 NZ_CP075320:2308-2586 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References