Detailed information of TA system TA167648

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 59274..59543
• Replicon: plasmid unnamed1
• Accession: NZ_CP058834
• Organism: Shigella flexneri strain STLEFF_27

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: HZT22_RS23675
• Protein ID: WP_001372321.1
• Coordinates: 59418..59543 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	59274..59339 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT22_RS23630	54382..54753	+	372	WP_223372515.1	hypothetical protein	-
HZT22_RS23635	54898..55104	+	207	WP_015059635.1	hypothetical protein	-
HZT22_RS23640	55130..55582	+	453	WP_063072992.1	single-stranded DNA-binding protein	-
HZT22_RS23645	55644..55877	+	234	WP_000006030.1	DUF905 family protein	-
HZT22_RS23650	55942..57900	+	1959	WP_063072993.1	ParB/RepB/Spo0J family partition protein	-
HZT22_RS23655	57955..58389	+	435	WP_000845907.1	conjugation system SOS inhibitor PsiB	-
HZT22_RS23660	58386..59105	+	720	WP_001276240.1	plasmid SOS inhibition protein A	-
HZT22_RS23665	59117..59305	-	189	WP_001299721.1	hypothetical protein	-
-	59117..59341	+	225	NuclAT_0	-	-
-	59117..59341	+	225	NuclAT_0	-	-
-	59117..59341	+	225	NuclAT_0	-	-
-	59117..59341	+	225	NuclAT_0	-	-
-	59274..59339	-	66	-	-	Antitoxin
HZT22_RS23670	59327..59476	+	150	Protein_76	plasmid maintenance protein Mok	-
HZT22_RS23675	59418..59543	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
HZT22_RS23680	59763..59993	+	231	WP_071845785.1	hypothetical protein	-
HZT22_RS23685	59991..60164	-	174	Protein_79	hypothetical protein	-
HZT22_RS23690	60234..60440	+	207	WP_063609967.1	hypothetical protein	-
HZT22_RS23695	60465..60752	+	288	WP_063072995.1	hypothetical protein	-
HZT22_RS23700	60871..61692	+	822	WP_001241365.1	DUF932 domain-containing protein	-
HZT22_RS23705	61989..62579	-	591	WP_222908884.1	transglycosylase SLT domain-containing protein	-
HZT22_RS23710	62982..63365	+	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
HZT22_RS23715	63552..64241	+	690	WP_000283379.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	cmlA1 / qacE / blaCTX-M-14 / erm(B) / mph(A)	-	1..98962	98962

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T167648 WP_001372321.1 NZ_CP058834:59418-59543 [Shigella flexneri]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T167648 NZ_CP075286:c4998982-4998065 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT167648 NZ_CP058834:c59339-59274 [Shigella flexneri]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin