Detailed information of TA system TA167645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4084456..4085150
• Replicon: chromosome
• Accession: NZ_CP058833
• Organism: Shigella flexneri strain STLEFF_27

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: HZT22_RS19865
• Protein ID: WP_001263489.1
• Coordinates: 4084752..4085150 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: HZT22_RS19860
• Protein ID: WP_000554758.1
• Coordinates: 4084456..4084749 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT22_RS19840 (4080088)	4080088..4080585	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
HZT22_RS19845 (4080809)	4080809..4082521	-	1713	Protein_3883	flagellar biosynthesis protein FlhA	-
HZT22_RS19850 (4082493)	4082493..4083278	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
HZT22_RS19855 (4083349)	4083349..4084404	+	1056	WP_001226164.1	DNA polymerase IV	-
HZT22_RS19860 (4084456)	4084456..4084749	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HZT22_RS19865 (4084752)	4084752..4085150	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HZT22_RS19870 (4085160)	4085160..4085612	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
HZT22_RS19875 (4085930)	4085930..4086136	+	207	Protein_3889	RtcB family protein	-
HZT22_RS19880 (4086132)	4086132..4086653	+	522	Protein_3890	peptide chain release factor H	-
HZT22_RS19885 (4086710)	4086710..4088167	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
HZT22_RS19890 (4088428)	4088428..4088886	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (4089482)	4089482..4089562	+	81	NuclAT_12	-	-
- (4089482)	4089482..4089562	+	81	NuclAT_12	-	-
- (4089482)	4089482..4089562	+	81	NuclAT_12	-	-
- (4089482)	4089482..4089562	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T167645 WP_001263489.1 NZ_CP058833:4084752-4085150 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T167645 NZ_CP075286:4128011-4128229 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT167645 WP_000554758.1 NZ_CP058833:4084456-4084749 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT167645 NZ_CP075286:4127610-4127984 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3