Detailed information of TA system TA167635

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1169042..1169544
• Replicon: chromosome
• Accession: NZ_CP058833
• Organism: Shigella flexneri strain STLEFF_27

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A8E0FJZ4
• Locus tag: HZT22_RS05910
• Protein ID: WP_000767833.1
• Coordinates: 1169042..1169296 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: HZT22_RS05915
• Protein ID: WP_001259255.1
• Coordinates: 1169293..1169544 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT22_RS05880 (1164057)	1164057..1164284	-	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-
HZT22_RS05885 (1164298)	1164298..1165356	-	1059	WP_000492324.1	thiosulfate utilization transporter TsuA/YeeE	-
HZT22_RS05890 (1165535)	1165535..1166893	-	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
HZT22_RS05895 (1166883)	1166883..1166945	-	63	WP_010723108.1	membrane protein YoeI	-
HZT22_RS05900 (1167160)	1167160..1168089	-	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
HZT22_RS05905 (1168135)	1168135..1168959	-	825	WP_000754758.1	SDR family oxidoreductase	-
HZT22_RS05910 (1169042)	1169042..1169296	-	255	WP_000767833.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
HZT22_RS05915 (1169293)	1169293..1169544	-	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
HZT22_RS05920 (1169827)	1169827..1169877	+	51	WP_001364200.1	his operon leader peptide	-
HZT22_RS05925 (1170023)	1170023..1170922	+	900	WP_000131756.1	ATP phosphoribosyltransferase	-
HZT22_RS05930 (1170928)	1170928..1172226	+	1299	WP_049290931.1	histidinol dehydrogenase	-
HZT22_RS05935 (1172229)	1172229..1173299	+	1071	WP_000108940.1	histidinol-phosphate transaminase	-
HZT22_RS05940 (1173299)	1173299..1174366	+	1068	WP_000080093.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10173.54 Da
• Isoelectric Point: 8.0353

>T167635 WP_000767833.1 NZ_CP058833:c1169296-1169042 [Shigella flexneri]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPESLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T167635 NZ_CP075256:c5117208-5116840 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGACAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT167635 WP_001259255.1 NZ_CP058833:c1169544-1169293 [Shigella flexneri]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT167635 NZ_CP075256:c5117426-5117205 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0