Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3637119..3637660 Replicon chromosome
Accession NZ_CP058831
Organism Shigella flexneri strain STLE4

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag HZT21_RS17765 Protein ID WP_000615983.1
Coordinates 3637382..3637660 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag HZT21_RS17760 Protein ID WP_000729703.1
Coordinates 3637119..3637379 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT21_RS17740 (3632794) 3632794..3633579 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
HZT21_RS17745 (3633551) 3633551..3635263 + 1713 Protein_3487 flagellar biosynthesis protein FlhA -
HZT21_RS17750 (3635487) 3635487..3635984 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
HZT21_RS17755 (3636160) 3636160..3636909 - 750 WP_240763318.1 NlpC/P60 family protein -
HZT21_RS17760 (3637119) 3637119..3637379 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HZT21_RS17765 (3637382) 3637382..3637660 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HZT21_RS17770 (3637816) 3637816..3638556 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
HZT21_RS17775 (3638527) 3638527..3639294 - 768 WP_000333380.1 class II glutamine amidotransferase -
HZT21_RS17780 (3639500) 3639500..3640078 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T167619 WP_000615983.1 NZ_CP058831:3637382-3637660 [Shigella flexneri]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T167619 NZ_CP075192:c2476047-2475742 [Ferribacterium limneticum]
ATGACCAAGAAAATTGACCCTGTGGGTCTGCCCGAATTTGATCTGGCCGAATATCTGGACAGCGAAGTGGCGATTTCGGA
GTACCTGAACCAGACTATTGCCGATGGCGACATGGCTGAGCTCGCCCACGCCCTGGGTGTGGCGGCGCGCGCTCGTGGCA
TGACCGAGGTGGCCAAGGCTTCCGGTATCACCCGGGAGGCGCTCTACAAGGCGCTGCGCCCGAATGCCAGCCCGCGTTTT
GAAACCGTCAGCAAGGTTTGTGCGGCACTCGGTGTTCGTCTGGTGGCGCAGCCGATCAATTCATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT167619 WP_000729703.1 NZ_CP058831:3637119-3637379 [Shigella flexneri]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT167619 NZ_CP075192:c2476349-2476053 [Ferribacterium limneticum]
ATGTATTCGATCAAAACCCTGCCTGAATTTACCGAGTGGCTAAGCGCTCTGAAGTACAAGTCGACGCTGATCCGCTTGGT
CCGTCGGTTGGACAAGGTTCAGCGTGGCAATCTTGGCGATGTGAAGGCGGTCGGTCATGGCGTATTCGAGATGCGTGAGC
ATTTCGGGCCGGGCTGGCGGATGTATTACGTGCAGCGCGGAGCGTTTTTGATTGTGATGCTGGGCGGTGGCGAAAAATCG
ACGCAGGCCGCCGATCTAGCCCGAGCCGTGAAGTTGGCGGCGACGATCAAGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References