Detailed information of TA system TA167618

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3630922..3631616
• Replicon: chromosome
• Accession: NZ_CP058831
• Organism: Shigella flexneri strain STLE4

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: HZT21_RS17725
• Protein ID: WP_001263489.1
• Coordinates: 3630922..3631320 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: HZT21_RS17730
• Protein ID: WP_000554758.1
• Coordinates: 3631323..3631616 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3626510)	3626510..3626590	-	81	NuclAT_11	-	-
- (3626510)	3626510..3626590	-	81	NuclAT_11	-	-
- (3626510)	3626510..3626590	-	81	NuclAT_11	-	-
- (3626510)	3626510..3626590	-	81	NuclAT_11	-	-
HZT21_RS17700 (3627186)	3627186..3627644	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HZT21_RS17705 (3627905)	3627905..3629362	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
HZT21_RS17710 (3629419)	3629419..3629940	-	522	Protein_3480	peptide chain release factor H	-
HZT21_RS17715 (3629936)	3629936..3630142	-	207	Protein_3481	RtcB family protein	-
HZT21_RS17720 (3630460)	3630460..3630912	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
HZT21_RS17725 (3630922)	3630922..3631320	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HZT21_RS17730 (3631323)	3631323..3631616	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HZT21_RS17735 (3631668)	3631668..3632723	-	1056	WP_001226164.1	DNA polymerase IV	-
HZT21_RS17740 (3632794)	3632794..3633579	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
HZT21_RS17745 (3633551)	3633551..3635263	+	1713	Protein_3487	flagellar biosynthesis protein FlhA	-
HZT21_RS17750 (3635487)	3635487..3635984	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T167618 WP_001263489.1 NZ_CP058831:c3631320-3630922 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T167618 NZ_CP075192:1642859-1643140 [Ferribacterium limneticum]
ATGCGGAAGATTGACCCGACCGGCCAATTCAGGCGCGACTACAAACGCGAGGCTAAGGGCCAGCATCGGGCAACGCTTGA
TGCCGAGCTGGAGCCGGTCTTGGAGGCTCTGGCTAGCGATCAGCCGCTAGAGCCTCGGCACCGCGACCATGCGCTTACGG
GCGAGTGGAAGGATCATCGGGATTGTCACGTAAAGCCTGACTTGGTGCTGATCTATCGAAAGCCAGATGACGTGACGTTG
CAACTCGTTCGGCTTGGCTCGCACAGCGAGCTTGGCCTGTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT167618 WP_000554758.1 NZ_CP058831:c3631616-3631323 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT167618 NZ_CP075192:1642609-1642872 [Ferribacterium limneticum]
ATGGCTAGCAATGCTGTTGTTCGTGCGCGCATCGATGAGCACATAAAGGAAGAGGCAAGCACCGTTCTTGCGGCAATGGG
GCTGACCGTCTCCGACGCCTTCCGCATCATGCTGACCCGTGTTGCCCGCGAGAAGGCATTGCCGTTCGAGCCTTTGATTC
CGAACGAAACCACCATCGCAGCCATGCGTGAGGCACGCGCCGGCAACCTCAAGAGCTTCGACAGCGTTGGGGCGTTGATG
GCGGACCTGCATGCGGAAGATTGA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3