Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3630922..3631616 Replicon chromosome
Accession NZ_CP058831
Organism Shigella flexneri strain STLE4

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag HZT21_RS17725 Protein ID WP_001263489.1
Coordinates 3630922..3631320 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag HZT21_RS17730 Protein ID WP_000554758.1
Coordinates 3631323..3631616 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3626510) 3626510..3626590 - 81 NuclAT_11 - -
- (3626510) 3626510..3626590 - 81 NuclAT_11 - -
- (3626510) 3626510..3626590 - 81 NuclAT_11 - -
- (3626510) 3626510..3626590 - 81 NuclAT_11 - -
HZT21_RS17700 (3627186) 3627186..3627644 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
HZT21_RS17705 (3627905) 3627905..3629362 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
HZT21_RS17710 (3629419) 3629419..3629940 - 522 Protein_3480 peptide chain release factor H -
HZT21_RS17715 (3629936) 3629936..3630142 - 207 Protein_3481 RtcB family protein -
HZT21_RS17720 (3630460) 3630460..3630912 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
HZT21_RS17725 (3630922) 3630922..3631320 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
HZT21_RS17730 (3631323) 3631323..3631616 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
HZT21_RS17735 (3631668) 3631668..3632723 - 1056 WP_001226164.1 DNA polymerase IV -
HZT21_RS17740 (3632794) 3632794..3633579 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
HZT21_RS17745 (3633551) 3633551..3635263 + 1713 Protein_3487 flagellar biosynthesis protein FlhA -
HZT21_RS17750 (3635487) 3635487..3635984 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T167618 WP_001263489.1 NZ_CP058831:c3631320-3630922 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T167618 NZ_CP075192:1642859-1643140 [Ferribacterium limneticum]
ATGCGGAAGATTGACCCGACCGGCCAATTCAGGCGCGACTACAAACGCGAGGCTAAGGGCCAGCATCGGGCAACGCTTGA
TGCCGAGCTGGAGCCGGTCTTGGAGGCTCTGGCTAGCGATCAGCCGCTAGAGCCTCGGCACCGCGACCATGCGCTTACGG
GCGAGTGGAAGGATCATCGGGATTGTCACGTAAAGCCTGACTTGGTGCTGATCTATCGAAAGCCAGATGACGTGACGTTG
CAACTCGTTCGGCTTGGCTCGCACAGCGAGCTTGGCCTGTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT167618 WP_000554758.1 NZ_CP058831:c3631616-3631323 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT167618 NZ_CP075192:1642609-1642872 [Ferribacterium limneticum]
ATGGCTAGCAATGCTGTTGTTCGTGCGCGCATCGATGAGCACATAAAGGAAGAGGCAAGCACCGTTCTTGCGGCAATGGG
GCTGACCGTCTCCGACGCCTTCCGCATCATGCTGACCCGTGTTGCCCGCGAGAAGGCATTGCCGTTCGAGCCTTTGATTC
CGAACGAAACCACCATCGCAGCCATGCGTGAGGCACGCGCCGGCAACCTCAAGAGCTTCGACAGCGTTGGGGCGTTGATG
GCGGACCTGCATGCGGAAGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References