Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1751899..1752401 Replicon chromosome
Accession NZ_CP058831
Organism Shigella flexneri strain STLE4

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HZT21_RS08290 Protein ID WP_000767829.1
Coordinates 1752147..1752401 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A6N8RC01
Locus tag HZT21_RS08285 Protein ID WP_001259251.1
Coordinates 1751899..1752150 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT21_RS08260 (1747076) 1747076..1748143 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
HZT21_RS08265 (1748143) 1748143..1749213 - 1071 WP_032082632.1 histidinol-phosphate transaminase -
HZT21_RS08270 (1749210) 1749210..1750514 - 1305 WP_032082631.1 histidinol dehydrogenase -
HZT21_RS08275 (1750520) 1750520..1751419 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
HZT21_RS08280 (1751565) 1751565..1751615 - 51 WP_001364200.1 his operon leader peptide -
HZT21_RS08285 (1751899) 1751899..1752150 + 252 WP_001259251.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HZT21_RS08290 (1752147) 1752147..1752401 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HZT21_RS08295 (1752484) 1752484..1753308 + 825 WP_000754737.1 SDR family oxidoreductase -
HZT21_RS08300 (1753354) 1753354..1754283 + 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
HZT21_RS08305 (1754498) 1754498..1754560 + 63 WP_010723108.1 membrane protein YoeI -
HZT21_RS08310 (1754550) 1754550..1755908 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HZT21_RS08315 (1756087) 1756087..1757145 + 1059 WP_000492340.1 thiosulfate utilization transporter TsuA/YeeE -
HZT21_RS08320 (1757159) 1757159..1757386 + 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T167605 WP_000767829.1 NZ_CP058831:1752147-1752401 [Shigella flexneri]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T167605 NZ_CP075190:c3188500-3188330 [Ferribacterium limneticum]
ATGCGCGTTGTCAATTTCTCCGATGCCGTCGTGATGTCGCTCGACACCTTCAACAGCCTCATGGAGACGGTCCAATTGCT
GAAATCGCCGGCCAACGCCGCCCATCTGGTGCGCTCTATCGAGCAATACCGCAAAGGTAAAACCGAGGCGCAGGATTTGG
CGGATGCCTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9293.56 Da        Isoelectric Point: 4.7815

>AT167605 WP_001259251.1 NZ_CP058831:1751899-1752150 [Shigella flexneri]
MRTISYSEARQNLSATMMKAVDDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT167605 NZ_CP075190:c3188337-3188071 [Ferribacterium limneticum]
ATGCCTAGTCAAATCACCTGGACCCTCGCCGCCTGGGAGGAAAACCAGTGCTGGCAAGGCCAGGACAGCAAGACGCTGAA
ACGGATCAACGCGGTCATCCGCGATTGTCTGCGCGAACCATTCGATGGCATCGGCAAACCCGAACCGCTGAAAGAGAACC
TCTCAGGCTTCTGGTATCGGCGTATCGACGATACCAATCGCCTTGTTTATTGCGTCGATGGGGGCAATCAGGTGATCATC
GCTTGCCGGTATCACTACGATGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A6N8RC01

References