Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 648261..648988 Replicon chromosome
Accession NZ_CP058831
Organism Shigella flexneri strain STLE4

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag HZT21_RS03170 Protein ID WP_000550189.1
Coordinates 648261..648575 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HZT21_RS03175 Protein ID WP_000560266.1
Coordinates 648572..648988 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT21_RS03150 (644428) 644428..645414 - 987 WP_023281494.1 Gfo/Idh/MocA family oxidoreductase -
HZT21_RS03155 (645493) 645493..646176 - 684 WP_001183042.1 vancomycin high temperature exclusion protein -
HZT21_RS03160 (646253) 646253..646756 - 504 WP_001295542.1 M48 family metallopeptidase -
HZT21_RS03165 (646841) 646841..647977 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
HZT21_RS03170 (648261) 648261..648575 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
HZT21_RS03175 (648572) 648572..648988 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
HZT21_RS03180 (649033) 649033..651051 - 2019 WP_049253702.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
HZT21_RS03185 (651377) 651377..653728 - 2352 WP_024238710.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T167601 WP_000550189.1 NZ_CP058831:648261-648575 [Shigella flexneri]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T167601 NZ_CP075188:3044470-3044751 [Quatrionicoccus australiensis]
ATGACGATACGTTCATTTCGCTGCACCGAAACTGAAGCCTTGTATGCGGGTGAAAGCTCTCGCCGTTTTCGCAATATCGA
AAGCGTCGCCCAGCGCAAGTTGCAAATGCAGGATGCTGCCGCCGAGTTGCGCGATCTGCGCGCGCCGCCCGGCAATCGCC
TGGAAGCACTGAGCGACGACCGGGCCGGACAGCACAGCATCCGCATCAACGAGCAGTGGCGGATATGCTTCAAATGGACT
GAGAATGGCCCGACCCGGGTTGAAATCGTCGACTATCACTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT167601 WP_000560266.1 NZ_CP058831:648572-648988 [Shigella flexneri]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT167601 NZ_CP075188:3044761-3045066 [Quatrionicoccus australiensis]
ATGGCGACCAACAATATGCGTGCGATCCACCCCGGCGAAATTCTCCGCGAAGAATTTCTGCTGCCGCTCGACATGAGCGC
CAACGCGCTTGCCATGGCCTTGCACGTTCCGGCCCCGCGCATCAACGACATCGTCCGCGAAAAGCGGGCGATCAGCCCCG
ATACCGCCTTGCGGCTCGCCCGTTATTTCGACACGACAGCGCTGTTCTGGCTCAACCTGCAAAGTACTTTCGACCTTAAG
CAGGCCGAGAACGAAAATGCCGAACGGATCGCCGAAGAAGTTCGCCCCTTGCAACGCGCTGCCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References