Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 604936..605735 Replicon chromosome
Accession NZ_CP058831
Organism Shigella flexneri strain STLE4

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag HZT21_RS02945 Protein ID WP_000347273.1
Coordinates 604936..605400 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HZT21_RS02950 Protein ID WP_001307405.1
Coordinates 605400..605735 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT21_RS02915 (599937) 599937..600371 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
HZT21_RS02920 (600389) 600389..601267 - 879 WP_001300474.1 PTS N-acetylgalactosamine transporter subunit IID -
HZT21_RS02925 (601257) 601257..602036 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
HZT21_RS02930 (602047) 602047..602520 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HZT21_RS02935 (602543) 602543..603823 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
HZT21_RS02940 (604072) 604072..604881 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HZT21_RS02945 (604936) 604936..605400 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HZT21_RS02950 (605400) 605400..605735 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HZT21_RS02955 (605884) 605884..607455 - 1572 WP_001723936.1 galactarate dehydratase -
HZT21_RS02960 (607830) 607830..609164 + 1335 WP_001723935.1 galactarate/glucarate/glycerate transporter GarP -
HZT21_RS02965 (609180) 609180..609950 + 771 WP_001723934.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 604936..616608 11672


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T167600 WP_000347273.1 NZ_CP058831:c605400-604936 [Shigella flexneri]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T167600 NZ_CP075188:c2989800-2989501 [Quatrionicoccus australiensis]
ATGATCGAAATCATCCAGAGCGAGACCTTCGCCCGGTGGCTGGGCAAGCTAAAGGACCGGTCCGCGGTTATGCGGATCAA
CGCCCGGCTTCGTCGCTTGACGGAGACCGGCAACTTCGGCGACGTGAAGCCTGTTCGCGAAGGTGTTAGCGAAATGAGGA
TCGACTACGGCCCTGGTTATCGTCTCTACTTTGTCCAGAACGGTCCGGTGTTGGTGGTCTTGCTCGCCGGTGGCGACAAG
AATACTCAGGATGCCGACATCAAACGTGCCATCGATATTTCCAAGGAGTGGAAGCCATGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT167600 WP_001307405.1 NZ_CP058831:c605735-605400 [Shigella flexneri]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT167600 NZ_CP075188:c2989504-2989193 [Quatrionicoccus australiensis]
ATGACAGAGAAATTTACCCGTTGGGACCCTGTTGATCAGTTGCTGAGTGGTGAAGACATGGCCCTCTACCTTGACGCCTG
CCTAGACGAAGACAACGGCGATGGAAGTGTGGTGCGTGCCGCTCTTAACGACATCGCCAGAGCGCAAGGCATGAGCCAGC
TTGCCCGTGACACTGGGCTGACTCGAGAGGGCCTGTACAAAGCATTGTCGCCAGCCGGCAATCCGGAGTTTTCGACGATC
CTGAAGGTTATCAAGGCGCTGAAAATTAAATTACATGCTACGCCTGGTGGTGACCACTTGGTCGCTGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References