Detailed information of TA system TA167582

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1830168..1830670
• Replicon: chromosome
• Accession: NZ_CP058828
• Organism: Shigella flexneri strain STLE3

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A8E0FJZ4
• Locus tag: HZT20_RS08550
• Protein ID: WP_000767833.1
• Coordinates: 1830416..1830670 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: HZT20_RS08545
• Protein ID: WP_001259255.1
• Coordinates: 1830168..1830419 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT20_RS08520 (1825346)	1825346..1826413	-	1068	WP_000080061.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
HZT20_RS08525 (1826413)	1826413..1827483	-	1071	WP_063114853.1	histidinol-phosphate transaminase	-
HZT20_RS08530 (1827480)	1827480..1828784	-	1305	WP_001442151.1	histidinol dehydrogenase	-
HZT20_RS08535 (1828790)	1828790..1829689	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
HZT20_RS08540 (1829835)	1829835..1829885	-	51	WP_001364200.1	his operon leader peptide	-
HZT20_RS08545 (1830168)	1830168..1830419	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
HZT20_RS08550 (1830416)	1830416..1830670	+	255	WP_000767833.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
HZT20_RS08555 (1830753)	1830753..1831577	+	825	WP_000754758.1	SDR family oxidoreductase	-
HZT20_RS08560 (1831623)	1831623..1832552	+	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
HZT20_RS08565 (1832767)	1832767..1832829	+	63	WP_010723108.1	membrane protein YoeI	-
HZT20_RS08570 (1832819)	1832819..1834177	+	1359	WP_024198437.1	putrescine/proton symporter PlaP	-
HZT20_RS08575 (1834356)	1834356..1835414	+	1059	WP_195714508.1	thiosulfate utilization transporter TsuA/YeeE	-
HZT20_RS08580 (1835428)	1835428..1835655	+	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10173.54 Da
• Isoelectric Point: 8.0353

>T167582 WP_000767833.1 NZ_CP058828:1830416-1830670 [Shigella flexneri]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPESLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T167582 NZ_CP075174:c3910390-3910103 [Salmonella enterica subsp. houtenae serovar 45:g,z51:-]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACTGTGTCCGCTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCCCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT167582 WP_001259255.1 NZ_CP058828:1830168-1830419 [Shigella flexneri]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT167582 NZ_CP075174:c3910116-3909814 [Salmonella enterica subsp. houtenae serovar 45:g,z51:-]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Structures

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0