Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 690602..691329 Replicon chromosome
Accession NZ_CP058828
Organism Shigella flexneri strain STLE3

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag HZT20_RS03360 Protein ID WP_000550189.1
Coordinates 690602..690916 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HZT20_RS03365 Protein ID WP_000560266.1
Coordinates 690913..691329 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT20_RS03340 (686760) 686760..687746 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
HZT20_RS03345 (687825) 687825..688517 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
HZT20_RS03350 (688594) 688594..689097 - 504 WP_001333820.1 M48 family metallopeptidase -
HZT20_RS03355 (689182) 689182..690318 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
HZT20_RS03360 (690602) 690602..690916 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
HZT20_RS03365 (690913) 690913..691329 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
HZT20_RS03370 (691374) 691374..693392 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
HZT20_RS03375 (693818) 693818..696169 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T167577 WP_000550189.1 NZ_CP058828:690602-690916 [Shigella flexneri]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T167577 NZ_CP075174:1836021-1836353 [Salmonella enterica subsp. houtenae serovar 45:g,z51:-]
ATGGACCGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCCGGTCACGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCCGTTGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCCCGCG
CAGCCGGTTTTACCGTCTCCCTGGACGGCGCGGGAACAAAAACAACGGGTGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGGCGCCCGGAGCGGTAAGCGGCTGGAACGTATACCCGATGCTGTAGTGAATGAAGTGCTGGCCCGACTGGAAGC
CATTCTGAGCTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT167577 WP_000560266.1 NZ_CP058828:690913-691329 [Shigella flexneri]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT167577 NZ_CP075174:1835763-1836020 [Salmonella enterica subsp. houtenae serovar 45:g,z51:-]
ATGTATACCACTCGCCTGAAAAAGGTTGGCGGATCCGTCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTTGGGCT
GTCAACCGACAGCGACGTGGGCATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCATTATTCGC
TTGAGGAACTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATCCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References