Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3512550..3513091 Replicon chromosome
Accession NZ_CP058826
Organism Shigella flexneri strain STLE1

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag HZT18_RS17155 Protein ID WP_000615979.1
Coordinates 3512813..3513091 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HZT18_RS17150 Protein ID WP_000729704.1
Coordinates 3512550..3512810 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT18_RS17130 (3508233) 3508233..3509018 - 786 WP_072132640.1 putative lateral flagellar export/assembly protein LafU -
HZT18_RS17135 (3508990) 3508990..3510702 + 1713 Protein_3365 flagellar biosynthesis protein FlhA -
HZT18_RS17140 (3510918) 3510918..3511415 - 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
HZT18_RS17145 (3511591) 3511591..3512364 - 774 WP_001353765.1 C40 family peptidase -
HZT18_RS17150 (3512550) 3512550..3512810 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HZT18_RS17155 (3512813) 3512813..3513091 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HZT18_RS17160 (3513247) 3513247..3513987 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
HZT18_RS17165 (3513958) 3513958..3514725 - 768 WP_000333379.1 class II glutamine amidotransferase -
HZT18_RS17170 (3514931) 3514931..3515509 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T167572 WP_000615979.1 NZ_CP058826:3512813-3513091 [Shigella flexneri]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T167572 NZ_CP075171:c2511004-2510810 [Leptodesmis sichuanensis PKUAC-SCTA121]
ATGAAAGTTCGAGATGTACTCAAACGGTTAGAAGCGGATGGTTGGTATCAAATCCGAATGCGAGGTAGCCATCGAATTCT
TGCCCATGAAACAAAGTCAGGAATCGTGGTTGTTCCTGGCAAGCCTGGAGACGAGATACCCAAAGGCACATTAGCATCGA
TTTGGAAACAAGCTCAATTGGAGGATGAATCATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT167572 WP_000729704.1 NZ_CP058826:3512550-3512810 [Shigella flexneri]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT167572 NZ_CP075171:c2510813-2510598 [Leptodesmis sichuanensis PKUAC-SCTA121]
ATGAAAGAGTATGCAGTTATTTATGAACAAGGCGAGACAAACTGGGGAGCAATCGTTCCCGACCTACCCGGTTGTGTGAG
CATTGGAGATACTTTTGAAGAAGTTCAGGAAAATGTTAAAGAAGCGATTGAACTTTACCTTGAGGTTTTGCAAGAACGCG
GGGAACCCATTCCAGAACCAACAACTAAAGTTGGATTGGTTGGAGTTGCTGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References