Detailed information of TA system TA167571

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3506361..3507055
• Replicon: chromosome
• Accession: NZ_CP058826
• Organism: Shigella flexneri strain STLE1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: HZT18_RS17115
• Protein ID: WP_001263493.1
• Coordinates: 3506361..3506759 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: HZT18_RS17120
• Protein ID: WP_000554757.1
• Coordinates: 3506762..3507055 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3502021)	3502021..3502101	-	81	NuclAT_12	-	-
- (3502021)	3502021..3502101	-	81	NuclAT_12	-	-
- (3502021)	3502021..3502101	-	81	NuclAT_12	-	-
- (3502021)	3502021..3502101	-	81	NuclAT_12	-	-
HZT18_RS17085 (3501361)	3501361..3502605	-	1245	WP_000189541.1	esterase FrsA	-
HZT18_RS17090 (3502697)	3502697..3503155	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HZT18_RS17095 (3503416)	3503416..3504873	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
HZT18_RS17100 (3504930)	3504930..3505451	-	522	Protein_3358	peptide chain release factor H	-
HZT18_RS17105 (3505450)	3505450..3505653	-	204	Protein_3359	RtcB family protein	-
HZT18_RS17110 (3505899)	3505899..3506351	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
HZT18_RS17115 (3506361)	3506361..3506759	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HZT18_RS17120 (3506762)	3506762..3507055	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HZT18_RS17125 (3507107)	3507107..3508162	-	1056	WP_001226164.1	DNA polymerase IV	-
HZT18_RS17130 (3508233)	3508233..3509018	-	786	WP_072132640.1	putative lateral flagellar export/assembly protein LafU	-
HZT18_RS17135 (3508990)	3508990..3510702	+	1713	Protein_3365	flagellar biosynthesis protein FlhA	-
HZT18_RS17140 (3510918)	3510918..3511415	-	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T167571 WP_001263493.1 NZ_CP058826:c3506759-3506361 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T167571 NZ_CP075171:c1654286-1654011 [Leptodesmis sichuanensis PKUAC-SCTA121]
ATGAAAATTTGGGTAGACGCACAGTTGTCTCCTGCGATCGCAACTTGGATTAGCAGCACTTTTGGAGTAACAGCATTAGC
TTTACGGGATATTGGTTTGAGAGACGCTGAAGATCCTGAAATCTTTGAGGCAGCAAAAGCTCAAGGCGTTATTTTCATCC
CACATTCCGCACTTTCAACTGGCACATCAGGGATTAACGACGCAGCCACGGACTCCCAGAAGCCTTGGCCAGAGATTTAT
CTCAATAAACAGCATCTATTCTCAACTGCGCTCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT167571 WP_000554757.1 NZ_CP058826:c3507055-3506762 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT167571 NZ_CP075171:c1654507-1654283 [Leptodesmis sichuanensis PKUAC-SCTA121]
ATGTCAGATTTGCCTAAAAGAATCACAGTTAATCCAAACCAATGTGGTGGTCGCCCTTGCATTCGTGGCATGAGAATTAG
AGTCTCGGATGTACTGGATTTATTTGCGGCTGGACTGAGTGCTGAGCAAATCTTGGAAGAGATGCCTGACTTAGAGGCCA
ATGATTTGAAGGCCGCACTTCTATATGCCTCACGGAAGCTGAATCATCCAGTGCTGGTCGCATGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6