Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 607002..607801 Replicon chromosome
Accession NZ_CP058826
Organism Shigella flexneri strain STLE1

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag HZT18_RS02945 Protein ID WP_000347251.1
Coordinates 607002..607466 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HZT18_RS02950 Protein ID WP_001307405.1
Coordinates 607466..607801 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT18_RS02915 (602003) 602003..602437 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
HZT18_RS02920 (602455) 602455..603333 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
HZT18_RS02925 (603323) 603323..604102 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
HZT18_RS02930 (604113) 604113..604586 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HZT18_RS02935 (604609) 604609..605889 - 1281 WP_000681905.1 tagatose-bisphosphate aldolase subunit KbaZ -
HZT18_RS02940 (606138) 606138..606947 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HZT18_RS02945 (607002) 607002..607466 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HZT18_RS02950 (607466) 607466..607801 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HZT18_RS02955 (607950) 607950..609521 - 1572 WP_001273753.1 galactarate dehydratase -
HZT18_RS02960 (609896) 609896..611230 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
HZT18_RS02965 (611246) 611246..612016 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T167554 WP_000347251.1 NZ_CP058826:c607466-607002 [Shigella flexneri]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T167554 NZ_CP075169:c1915957-1915706 [Sodalis ligni]
TTGCGCCTGTTTTTCGAGAAAGGATCGACTGCGGGTATTGATGCGAAGCAGTCCGGTAAACTCCGCCTACGTCTGGCGGT
CCTCGATGCAGCGGAAGAGATCAAGGATATTGACCGACCGGGATATTCCCTCCATACGCTTACCGGGGATAGGAAAGGCC
AATGGTCAATAACCGTCACGGGCAACTGGCGCATTACGTTTGAATTTATGGATGGCAACGCCTATATCGTTAACTATGAG
GATTATCACTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT167554 WP_001307405.1 NZ_CP058826:c607801-607466 [Shigella flexneri]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT167554 NZ_CP075169:c1915706-1915419 [Sodalis ligni]
ATGACTATGTTCAATCCGCCGCACCCCGGTGAAATCATTGCAGAGACCCTGGACGATCTGAATATCGGCATTCGTGAGTT
ATCCCGAGCATTGCATGTCGCCCCTTCTACAGTACAACGTCTGGTTAGCGGTCATTCTGCGGTTTCGCCAGAAATGGCCC
TAAAACTTTCGGCGGTGCTTGGTAGTTCGGCAAAGTTTTGGCTCAATCTTCAAGATAATTACAGCCTTTGGGCGGCGCAA
AAAGAAGTTGATATCTCAAAGCTGCACCGGTTGGTTGCAGTTGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References