Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3789764..3790305 Replicon chromosome
Accession NZ_CP058796
Organism Shigella flexneri strain STLIN_8

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag HZT12_RS18870 Protein ID WP_000615979.1
Coordinates 3790027..3790305 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HZT12_RS18865 Protein ID WP_000729704.1
Coordinates 3789764..3790024 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT12_RS18845 (3785447) 3785447..3786232 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
HZT12_RS18850 (3786204) 3786204..3787916 + 1713 Protein_3701 flagellar biosynthesis protein FlhA -
HZT12_RS18855 (3788132) 3788132..3788629 - 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
HZT12_RS18860 (3788805) 3788805..3789578 - 774 WP_001353765.1 C40 family peptidase -
HZT12_RS18865 (3789764) 3789764..3790024 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HZT12_RS18870 (3790027) 3790027..3790305 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HZT12_RS18875 (3790461) 3790461..3791201 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
HZT12_RS18880 (3791172) 3791172..3791939 - 768 WP_000333379.1 class II glutamine amidotransferase -
HZT12_RS18885 (3792145) 3792145..3792723 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T167551 WP_000615979.1 NZ_CP058796:3790027-3790305 [Shigella flexneri]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T167551 NZ_CP075169:964923-965348 [Sodalis ligni]
GTGGCCCTCTGGCGCTGTGATGTAAGGGTGTCGTGGCGCACGCAAATCTTCTCCCTGGTGATTCACGGGATATTGACGCT
ATTGATTCTGTTATCCCCCTGGCCCGAGAACTACGGTCTGGTTTGGGTCACGCTTATTACGCTTGTGGTCTTCGAGTGCA
TTCGCAGTCAGAGAAATATCATGGCCTGTCGGGGGGAACTCGTGCTGCAAAGCGGCCTGCACCTGCGCTGGCATAATAAA
GACTGGTTAATCAGGGGAACGCCCTGGATGCTGAAAAGCGGCATCCTGATCTCGTTACGCCAGGTGGGGGGAAGGCGCAC
ACGCAAACTCTGGCTGGCGGCGGACAGCATGGACCAGCGCGAATGGCGCAGCCTGCGCCAGGCTTTGCTGCATGCCGCCT
CGGTGCAACAGCGGCACGATCGTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT167551 WP_000729704.1 NZ_CP058796:3789764-3790024 [Shigella flexneri]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT167551 NZ_CP075169:964676-964942 [Sodalis ligni]
ATGGATATCGATAATAGAGCCCGCATCCACTGGGCCTGCCGCCGGGGCATGCGTGAACTGGATATCTCGATAATGCCGTT
TTTTGAATACGATTTCGACACCCTGAGCGACGAGGACAAAGGTTTGTTCGTGCGTCTGCTGGAATGCGACGACCCGGATC
TTTTCAACTGGCTGATGAATCATGGCGAGCCCCAGGATAGCGGATTGAAAAAAATGATTAACCTGATACAAACACGAAAT
AAAGCCCGTGGCCCTCTGGCGCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References