Detailed information of TA system TA167529

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 603628..604427
• Replicon: chromosome
• Accession: NZ_CP058796
• Organism: Shigella flexneri strain STLIN_8

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: S1NYM6
• Locus tag: HZT12_RS02975
• Protein ID: WP_000347251.1
• Coordinates: 603628..604092 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: HZT12_RS02980
• Protein ID: WP_053893542.1
• Coordinates: 604092..604427 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT12_RS02945 (598629)	598629..599063	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
HZT12_RS02950 (599081)	599081..599959	-	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
HZT12_RS02955 (599949)	599949..600728	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
HZT12_RS02960 (600739)	600739..601212	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
HZT12_RS02965 (601235)	601235..602515	-	1281	WP_000681905.1	tagatose-bisphosphate aldolase subunit KbaZ	-
HZT12_RS02970 (602764)	602764..603573	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
HZT12_RS02975 (603628)	603628..604092	-	465	WP_000347251.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
HZT12_RS02980 (604092)	604092..604427	-	336	WP_053893542.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
HZT12_RS02985 (604576)	604576..606147	-	1572	WP_001273753.1	galactarate dehydratase	-
HZT12_RS02990 (606522)	606522..607856	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
HZT12_RS02995 (607872)	607872..608642	+	771	WP_001058209.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17777.14 Da
• Isoelectric Point: 9.4947

>T167529 WP_000347251.1 NZ_CP058796:c604092-603628 [Shigella flexneri]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T167529 NZ_CP075140:c4904698-4904324 [Salmonella enterica]
ATGGTCAAAGGGTCTGCGCTTTTTGATACCAATATCCTCATCGACCTGTTTAGCGGTCGTATTGAAGCGAAACATGCGCT
GGAAGCGTATCCGCCACAGAATGCGATTAGCCTTATCACATGGATGGAAGTGATGGTGGGCGCTAAGAAATATCATCAGG
AAAACCGCACGCGAATTGCATTAAGTGCGTTTAATGTTATTGGTGTGACACAAGAAATCGCGGAACGAAGCGTCATTGTC
AGGCAGGAGTATGGCATGAAACTCCCGGATGCGATCATTCTGGCCACTGCACAAGTCCATCGTTGTGAGCTTGTGACGCG
TAATACAAAAGACTTTGCCGGCATTCCAGGAGTGATCACGCCGTATCACTTGTAG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12344.87 Da
• Isoelectric Point: 4.6647

>AT167529 WP_053893542.1 NZ_CP058796:c604427-604092 [Shigella flexneri]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLNPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT167529 NZ_CP075140:c4904940-4904698 [Salmonella enterica]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGACGATGTGATTAAACGGCTTGACGATCTCAAGGT
GCAACGCAACCTACCTCGTGCAGAACTCTTGCGGGAGGCTGTTGAGCAGTATCTGGAAAGACAGGATCGGGCTGAGACCA
CAATTTCGAAAGCATTGGGGCTCTGGCAAGGCTGTGAGGAAGATGGCGTTGAATACCAACGTAAACTTCGTGAGGAATGG
TAA

Structures

Toxin

• AlphaFold DB: A0A829CJ20